33 
peroxide, using 2 c. c. of the reagent and 0.1 c. c. of the solution of 
blood or of blood and tissue. After a short interval all of these 
tissues showed a retarding influence on the oxidation. Arranged in 
order of the amount of phenolphthalein formed as shovm by the 
color of the tubes, these solutions stood in the following order: 
Blood. 
Liver = Bone marrow = Lung = Siiprarenals. 
Spleen. 
Pancreas. 
Brain = Muscle. 
The greatest retardation was shown by the muscle and brain. At 
the end of fifteen minutes the tube containing blood alone was deep 
purplish red, whereas tubes (6) and (9) were only faint pink. When 
compared in the colorimeter with standard phenolphthalein, these 
three tubes gave the following readings: 
[Dec. 17, 1908.] 
Tube. 
Contents. 
Colorimeter scale 
readings. 
Test. 
standard. 
Number (1)... 
Blood 
5 
2.0 
Number (6) 
Blood and muscle 
5 
.2 
Number (9) 
Blood and brain 
5 
.4 
On standing one hour solutions (3), (5), and (7) showed even a 
greater coloration than (1). It should be borne in mind, however, 
that these three tissues, bone marrow, spleen, and lung, are highly 
vascular, and hence solutions (3), (5), and (7) probably actually 
contained somewhat more blood than solution (1). 
The conduct of these solutions was also tested towards alkaline 
phenolphthaiin alone, using 2 c. c. of the reagent and 1 c. c. of each 
of the solutions. These tests were allowed to stand twenty-four 
hours at ordinary temperature, when the colors were compared with 
the phenolphthalein standard, with the following results: 
Tube. 
Contents. 
: i 
Colorimeter scale ! 
readings. 
Test. 
Standard. ‘ 
1 
Number (1) 
Blood 
5 
1 
3.4 
Number (2) 
Blood and liver 
5 
1 
1.4 i 
Number (3).. . . 
Blood and marrow. . 
5 
1.7 
Number (4) 
Blood and pancreas 
0 
Number (5) 
Blood and spleen 
5 
2.0 
Number (6) 
Blood and muscle 
5 
.1 
Number (7) 
Blood and lung 
5 
1.7 
i Number (8) 
Blood and suprarenals 
5 
(?)4.2 
j Number (9) 
Blood and brain j 
5 
.4 
