115 
If agglutination occurs with either the t 3 rphoid or paratyphoid 
serum, agitate the tube to break up clumps and plate out some of 
the growth either on Endo or lactose-litmus agar plates. Xote the 
appearance of the colonies after 24 hours. Fish one or more of these 
colonies and so obtain the organism in pure culture. Then carry the 
organism through the yarious cultural and other tests required to 
establish positiyely its identity. 
DIRECTIOXS FOR MAKING ENDOWS MEDIFAI.® 
Make 4 per cent neutral agar as follows: Take 1 liter distilled 
water, add 5 grams sodium chloride, 10 grams Liebig’s extract meat, 
10 grams peptone. 
Dissolye by heating, allow to cool, and place on surface 40 grams 
powdered agar. When this has settled, place in Arnold sterilizer, 
coyering beaker with paper, and cook three hours. 
Filter through cotton on a perforated funnel (Buchnerfilter) by 
the aid of a yacuum or allow to settle while slowly cooling and dis- 
card the bottom turbid stratum. 
Neutralize to litmus paper with XagCOg solution. 
Add 10 c. c. sterile 10 per cent XagCOg solution. 
It is conyenient at this point to put up the medium in 100, 200, 
and 400 c. c. quantities in flasks, the flasks being large enough to 
proyide room for the other ingredients. These can be stored until 
needed for use ; then the agar is melted and the other ingredients added 
in the proper proportion. 
Add 10 grams chemically pure lactose. It is important that this 
should be pure. 
Add 5 c. c. alcoholic fuchsin solution. This is prepared as follows: 
To 10 grams fuchsin (not acid fuchsin) add 100 c. c. 96 per cent alcohol, 
shake, allow to stand 20 hours, and filter the supernatant fluid, which 
is used. It should be filtered each time before using. 
Add 25 c. c. freshly made and sterilized 10 per cent sodium sulphite 
solution. 
Sterilize for a few mmutes m the Arnold and pour plates while the 
medium is steaming hot. 
The medium after cooluig should be nearly colorless to transmitted 
light, and rose or flesh colored to reflected light. 
« References. 
Endo, S, Centblt. f. Bakt., yoI. 35, Xo. 1, 1903-4, p. 109. 
Klinger. Arb. a. d. Kais. Ges.-Amt., 1906, p. 52. See also Herford. 
Willson. Jour, byg., 1905, p. 429. 
Scheller. Centblt. f. Bakt., Sept. 15, 1906, p. 62. 
Clauditz. Hyg. runds., vol. 14, 1904, p. 718. 
Herford. Arb. a. d. Kais. Ges.-Amt., 1906, p. 62. 
Kayser. Munch, med. Woch., 1906, p. 17-18. 
