148 
agglutination in liigh dilution with antityphoid serum make it appear 
probable that this organism was the typhoid bacillus. 
Later in the season one of us (Lumsden) isolated from human 
feces an organism, culturally the colon bacillus, which would aggluti- 
nate quite readily in dilutions of 1 to 300 with this same (rabbit’s) 
antityphoid serum. A number of other strains of B. coli were then 
tried with this serum, but all failed to agglutinate in dilutions as low 
as 1 to 20. There seemed to be some specific relation between the 
organism and this jDarticular rabbit’s serum dependent upon some 
agglutinin other than typhoid in the serum, because two serums 
obtained from other animals — one rabbit and one horse — both failed 
to agglutinate the organism. 
The positive findmg in the case of C. H. J. has some possible 
clinical support in the fact that during the five or six weeks previous 
he had been indisposed — had sy^mptoms suggesting the possibility^ of 
a typhoid cholecystitis. 
2. Specimen of feces from C. E.: On the day^ this specimen was 
examined 41 other collected specimens of feces and 4 control speci- 
mens of feces containing typhoid culture were examined, all on the 
same table. With such a large number of plates to handle there was 
a possibility, but we do not say^ a probability^, of error. 
One of the 4 control specimens containing 5 drops of typhoid culture 
freshly added examined that day was reported negative. Identity 
of organism isolated established by full cultural study^ and by agglu- 
tination in high dilution with two antityphoid serums obtained from 
different animals. 
3. Specimen of urine from J. K. : The day^ this specimen was 
examined, 19 collected specimens of feces, one other collected speci- 
men of urine, and 4 control specimens of feces containing typhoid 
culture were examined. 
The Endo plates from this urine presented a large number and 
quite a variety^ of colonies, giving the plates more the appearance 
of having been made from feces than from urine. This probably was 
due to the urine having been collected in a vessel actually soiled 
with fecal matter. Therefore the typhoid bacilli isolated from this 
specimen may have come originally from the feces of this boy or from 
that of some other member of the family^. All the control specimens 
examined that day were reported positive. 
Identity of the organism isolated was established by complete 
cultural study and by its high agglutinability with two different 
antityphoid serums. Examinations of three specimens of urine and 
of three specimens of feces subsequently obtained from this boy were 
all negative for B. typhosus. Repeated examinations of the feces 
and urine of other members of the family were negative for B. 
typhosus. 
