19 
Table V. — Perfusion of the isolated frog^s heart with acetanilide and with acetanilide 
and sodium bicarbonate in Ringer's solution. 
Protocol 35, October 15, 1908. 
Protocol 38, October 16, 1908. 
Time. 
Rate. 
Output 
per 
5 minutes. 
Time. 
Rate. 
Output 
per 
5 minutes. 
i 3.20.... 
! 3.25.... 
1 3. 30. . . . 
i Aceta: 
T 
3.32.. .. 
3.35.. .. 
3.40.. .. 
3.45.. .. 
3.50.. .. 
Acet£ 
i 3.52.... 
1 3. 55 
4.00. ... 
4.03.. .. 
C. c. 
34 
46 27 
40 30 
nilide J per cent 4- 
^ NaHCOs. 
32 
18 19 
17 15 
17 12 
17 . 13 
irulide | per cent. 
14 
10 11 
7 3 
0 , 
1 
1. 55 
2.00 
2.05 
Acetanili 
cent+5 
NaHC( 
2.07 
2. 10 
2.20 
2.28 
Acetanili 
cei 
2. 30 
2.35 
42 
38 
33 
de 1 per 
c per cent 
h- 
21 
1 22 
22 
I 21 
de J per 
Qt. 
16 
14 
_Xot re- 
corded. 
Action on the dog^s heart. — The effect of acetanilide upon the mam- 
malian heart is considered such an important factor in the poisoning 
in man that the results of the control experiments done in determin- 
ing the nature of the effect of acetanilide caffeine combinations will 
be described in detail. Evans,® in describing the effect of acetanilide 
upon the circulation, states that it caused a rise of pressure and a 
slight acceleration in the heart rate when given to rabbits in doses of 
15 to 75 milligrams. Lepine^ reported in experiments on dogs that 
there was an increased heart rate, increased energy, and greater ten- 
sion in the arteries, but that this was followed by slowing and lessened 
tension. Hare‘S also worked with dogs and found a slight fall in pres- 
sure, and Osier‘S in clinical observations noted a decrease of the pulse 
of from 20 to 30 beats per minute. Weil® seems to have summed up 
the action of the drug in his experiments by stating that the first 
effect was an increase of rate and energy, but that this was followed 
by a decrease in both. It is generally concluded from experimental 
evidence, therefore, that small doses increase the heart action, but 
that larger amounts cause depression. Xo experiments seem to have 
been made in which the heart action of acetanilide was recorded by 
a myocardiograph. Accordingly it seems worth while to again report 
upon the action of this drug upon the circulatory organs as a means 
of emphasizing its dangers, especially as myocardiograph tracings 
demonstrate this action so clearly. 
It is generally recognized that the mammalian heart reacts to 
drugs and poisons in much the same manner as does the heart of cold- 
a Evans, Therap. Gazette, 1887, XI, 237. 
6 Lepine, Rev. de Med., Par., 1887, VII, 310. 
cHare, Therap. Gazette, 1887, XI, 382. 
Osier, Ibid., 165. 
« Weil, Paris Thesis, 1887, De la Acetanilide. 
