42 
lived 6, 6, 7, 10 days, an average of 7.25 days. The control mice 
fed on acetanilide alone, 0.050 gram per cake, lived 6, 6, 8, and 8 
days, an average of 7 days. These results show that salicylic acid 
neither increases nor decreases the toxic effect of acetanilide. 
TOXICITY OF ACETANILIDE AND SODIUM BROMIDE. 
Mice fed upon a mixture of acetanilide 0.050 and sodium bromide 
0.030 gram per cake hved approximately as long as the control, an 
average of 8 days, the controls an average of 8.25 days. This indi- 
cates no alteration in toxicity. 
The method of feeding mice upon cakes gives the results from long 
continued use of a drug, but the amount taken is entirely dependent 
upon the appetite of the animal. Therefore, to confirm the above 
residts, to make more certain the exact amount of drug given, and 
to test the action of acetanihde mixtures when given in amounts 
sufficiently large to produce acute poisoning, the following experi- 
ments were carried out upon guinea pigs. These animals were all of 
about the same weight, obtained from the same lot, and were kept 
under the same conditions. The dose was estimated per gram of 
body weight, each dose being weighed separately and made into pffis 
of suitable size with mucilage of acacia and arrow-root starch. These 
were dried until a shght crust was formed on the outside and then 
fed hi such a manner that none of the drug was lost during adminis- 
tration. The protocols in the following table show the toxicity of 
acetanilide as determined by this method of administration: 
[Acetanilide control: Determination of the minimum lethal dose of acetanilide for guinea pigs. — =sur- 
Tived; A = death.] 
Weight 
in 
grams. 
Dose per 
gram, weight 
in grams. 
Eesult. 
Hours 
tiU 
death. 
515 
0. OOOS 
490 
.0010 
— 
500 
.0010 
450 
.0010 
__ 
500 
.0010 
— 
14 
465 
.0012 
— 
445 
.0012 
— 
422 
.0014 
-L 
lOi 
4S5 
.0014 
-r 
12 
406 
.0016 
9i 
465 
.0016 
fi- 
32 
402 
.0018 
4 - 
12 i 
