47 
The use of sodium bicarbonate® has been suggested to relieve the 
gastric irritation which sometimes follows the use of antipyrine. 
Accordingly a series of perfusion experiments were carried out, using 
sodium bicarbonate in conjunction with antipyrine. The effect was 
to lessen but not abolish the poisonous action of antipyrine, both rate 
and output being increased to a considerable degree, although never 
to normal. The following protocols illustrate this antagonism; 
Table III . — Perfusion of the isolated frog’s heart with antipyrine and with antipyrine 
and sodium bicarbonate in Ringer’s solution. 
Protocol 81. 
Protocol 85. 
Output 
Output 
Time. 
Rate. 
per 
Time. 
Rate. 
per 
5 minutes. 
5 minutes. 
C. c. 
C. c. 
10. 35 
35 
Antipyrine 
3. 20 
32 
Antipyrine 
0.8 per cent. 
J per cent. 
10. 40 
24 
20 
3. 50 
23 
26 
10. 45 
24 
13 
3. 53 
21 
27 
10. 50 
24 
8 
4.00 
21 
27 
Antipyrine 0.8 
per cent 
Antipyrine per cent 
+ NaHCOs 4^ per cent. 
+ NaHCOs 4x5 per cent. 
10. 52 
28 
4.02 
24 
10. 53 
32 
4. 05 
22 
32 
10. 55 
25 
14 
4. 10 
19 
30 
11.00 
28 
17 
4.15 
20 
32 
11. 15 
27 
16 
4. 20 
21 
30 
Antipyrine h per cent. 
4. 22 
17 
4. 25 
16 
21 
4. 29 
0 
ACTION ON THE MAMMALIAN HEART. 
The changes in the dog’s heart induced by antipyrine injections 
were also determined, using the same methods as for acetanilide, 
except that the perfect solubility of antipyrine made its injection as 
an emulsion with acacia unnecessary. As in the perfusion experi- 
ments upon the frog’s heart, antipyrine was found to be very much 
less poisonous to the dog’s heart than acetanilide. Small doses 
(0.5 to 1 gram) were practically devoid of any depressant action, 
and a dose of 0.500 gram was actually stimulant, increasing the 
amplitude 15 millimeters through an increase in the completeness of 
the systole. Doses of 1 gram also increased the amount of contrac- 
tion, but the diastole was less complete, so that the result was a 
slight decrease in the amplitude. The injections of still larger 
amounts produced a very definite decrease in the heart’s action, 
but the change was not at all comparable to the depression following 
acetanilide in the same dose. Figure 7 is given to show the stimu- 
lant action of 1 gram antipyrine (compare this with the depressant 
action of 1 gram acetanilide. (Fig. 1, p. 21.) 
a Am. J. Pharm., 1888 , XVIII, 180 . 
