55 
movements. After about twenty minutes this was followed by less- 
ened movements, the animal sitting quietly and acting as if cold. 
The fur was roughened and occasionally slight convulsions appeared. 
Xo difference in the symptoms of poisoning could be determined in the 
two series of experiments. The toxicities of the mixture and of the 
compound, salipyrin, were also approximately the same as is shown 
by the protocols given in the following table: 
Series I. 
[Effect of salipyrin and of an antipyrine-salicylic acid mixture upon white mice, hypodermic injection.] 
Dose per gram 
body weight, 
in' grams. 
Salipyrin, 
A. and S. 
mixture. 
Hours till 
death. 
Hours till 
death. 
0. 0010 
0 
0 
.0011 
0 
3.39 
.0012 
2.33 1 
2. 47 
.0014 
1.25 ' 
1.03 
Series II. 
Dose per gram 
body weight, 
in grams. 
Salipyrin. 
A. and S. 
mixture. 
Hours till 
death. 
Hours till 
death. 
0. 0013 
2. 12 
1.44 
.0013 
2. 21 
2. 27 
.0013 
3. 02 
2^50 
Feeding experiments were also carried out to determine the toxic 
effect of salipyrin and of the antipyrine-salicylic acid mixture when 
given in a manner more closely simulating therapeutic administration. 
Guinea pigs of about the same weight and belonging to the same lot 
were used in the first series. The dose was estimated per gram body 
weight, each dose being weighed separately and made up into pills 
of suitable size and then fed in such a manner that none of the drug 
was lost in their administration. The results of the first series, using 
0.0016 milligram per gram body weight, indicated that salipyrin was 
most toxic, two pigs receiving salipyrin dying after ten and twelve 
hours, respectively. Two days later a second dose was administered 
in the same manner to the surviving animals, using, however, 0.0018 
milligram of the combination or of the mixture per gram body weight. 
In this series the toxicities appeared to be approximately the same. 
