18 
In tlie following table (Table XIV) are given the results obtained 
with solutions of varying amounts of alkaloid dissolved in an excess 
of hydrochloric acid, the amount of alkaloid, as well as the amount 
of hydrochloric acid being both unknown to the writer at the time 
when the determinations were carried out. 
Table XIV. 
(Unknown solutions submitted by Prof. J. H. Kastle.) 
Showing the proportioiiaUty between the NjlO NaOH equivalent of the amount of alka- 
loid given and that found to require. 
Temperature, 90-95 C.; time, 6 hours. 
Name of alkaloid. 
Amount 
given. 
X, 10 XaOH 
equivalent 
of amount 
given. 
X/10 XaOH 
required. 
Ratio I'e- 
tween the 
X;i0 XaOH 
equivalent 
and that 
found to re- 
quire. 
Gram. 
cc. 
cc. 
Cinchonine 
0. 0770 : 
5. 24 
5. 20 
1. 01 
Do 
. 1294 i 
8. 80 
&76 
1.00 
Do 
.2040 
18. 00 i 
18.00 
1.00 
Do... 
. 0670 
4. 60 
4. 43 
1.04 
Do 
. 1940 
13. 20 
la 10 
1.01 
Do.... 
.1617 
11.00 
10.89 
1.01 
strychnine 
. 1403 
4. 25 
4.09 
1.04 
‘Do 
. 2939 : 
S. SO 
8.76 
1.00 
Do 
. 0835 
2. 50 
2. 42 
1.03 
Do 
. 1937 
5.80 
5. 59 
1.04 
Do 
. 2505 
7. 50 
7. 18 
1.04 
Do 
. 1136 : 
3. 40 
3. 26 
1.04 
Average 
1. 02 
The results given in the accompanying tables appear, in the cases 
where hydrochloric acid was used as the volatile acid, sufficiently 
close to what is theoretically recjuired as to justify the conclusion 
that the small deviation from the theory in some cases is due largely 
to experimental error, and hence that the alkaloids, the study of which 
is here presented, may be estimated with fairly close results by the 
procedure here adopted, whether we use the alkalimetric method 
with the aid of phenolphthalein or determine the chlorine by means 
of Yolhard’s thiocyanate method. For estimating the actual amount 
of alkaloid in specimens of these alkaloids we may therefore adopt 
the following procedure : 
About 0.2 gm. of the specimen to be examined is dissolved in an 
excess of dilute hydrochloric acid (about 20 cc. of 4 per cent), the 
liquid completely evaporated, any film which may have formed over 
the surface is broken up with the aid of a glass rod, and the residue 
allowed to remain on the water bath (90-95 C.) for three hours. 
It is then taken up with distilled water (10-20 cc.), phenolphthalein 
(3 drops) solution added, and titrated with standard sodium hydrox- 
ide until the solution develops a light pink color. At this point one 
