11 
to the ones discussed in this paper. The work, however, did not 
attract much attention at the time and seems to have been lost sight 
of, their physiological activity not having been tested by him. 
Even Stolz, who was familiar with the work, does not give Dzierz- 
gowski and his contemporaries the credit they deserve for their pioneer 
work with these compounds. Among some of the compounds 
s}mthetized by Dzierzgowski are dimethyl-amido-aceto-catechol, 
anilido-aceto-catechol, methyl-anilido-aceto-catechol, quinolin-, pyri- 
din-, and piperidin- aceto-catechol, aceto-chloro-aceto- and chlor- 
propio-c at echol . 
Gluzihski (34) (1895) removed aseptically the adrenals of recently 
killed oxen, calves, hogs, rabbits, and dogs; weighed and ground 
them up vdth broken glass. One part of the pulp was allowed to 
macerate eight to twelve hours in a cold 50 per cent aqueous solution 
of glycerine. The solution was filtered through sterilized glass wool 
and the clear extract injected from a sterilized syringe into the ear 
vein of a rabbit. In one minute 0.3 to 1 gm. killed a 1,500 gm. 
rabbit. The extract heated to 100° for one hour retained its tox- 
icity, causing hemiplegia, loss of sensibility, cramps of anterior part 
of body, opisthotonus, rapid breathing, dilation of the pupil, and, 
finally, symptoms of dyspnoea, general paralysis, and, unless artificial 
respiration were admuiistered, death from asphyxia. 
Moore, B. (59) (1895), basing his opinion on experiments which 
indicated that such chemical operations as destroy the color reac- 
tions by oxidizing the reducing agent likevdse alter the physio- 
logical activity of the extract, concluded that Yulpian^s chromogen 
and the active principle are identical. 
Dubois (25) (1896) used an extract of fresh suprarenals of rats 
ground in equal volumes of alcohol and distilled water and allowed 
to remain twenty-four hours in glycerine. He concludes that such 
extracts contain two substances, one soluble in alcohol at 90°, 
causing vaso-dilation and congestion; the other, very soluble in this 
reagent, causing paralysis, depression of the heart, and death from 
asphyxia. 
Frankel (33) (1896) separated what he supposed to be the active 
principle, calling it ^^sphygmogenin, ’’ and observed that a close 
relation existed between the ease with which the substance is oxi- 
dized and its physiological activity. He suggested that ‘Aphygmo- 
genin” is a pyrocatechin derivative, and although he and Kruken- 
berg were in a measure correct it is now knovm that FrankeFs product 
was a mixture. 
Vincent, S. (73) (1897), performed a series of 80 experiments with 
rabbits, guinea pigs, rats, mice, frogs, and toads. The extract was 
made by boilmg a short time and filtering the chopped-up gland, 
although a dried gland, glycerine, or alcoholic extract was some- 
