13 
of Hg. Hunt maintains that these results justify AbePs contention 
that epinephrin is the active principle. 
Cybulskiego (19) found that 1 c. c. of a 10 per cent solution of the 
extract injected into the vein of a rabbit caused death, but if this 
solution were diluted ten to twenty times the same dose of adrenalin 
was borne without any untoward effects. 
Bouchard and Claude (14) (1902) experimented with a small num- 
ber of animals, finding that the lethal dose may be only 0.5 or 0.2 mg. 
per kilo, an animal occasionally withstanding an intravenous injec- 
tion of as much as 1 mg., 2 mg. usually proving fatal. The lethal dose 
for rabbits therefore lies between 1 and 2 mg. per kilo. Provided 
there was a gradual increase of each successive dose until a maximum 
was reached, they could inject as much as 4 mg. per kilo without any 
untoward effects other than temporary paresis such as is brought on 
by an initial dose of 1 mg. 
Battelli (7) (1902), using a slight modification of Takamine’s 
method, claims to have secured adrenahn even more pure than that 
obtained by Takamine. Battelli (9) injected this preparation sub- 
cutaneously into rabbits, guinea pigs, and frogs with the following 
results: With a corresponding number of guinea pigs 10 mg. per 
kilo proved to be the lethal dose. On the other hand, frogs were 
ten times more resistant than rabbits, 1,000 mg. killing only three 
out of four. 
Dose per 
kilo. 
Number of 
rabbits in- 
jected. 
Number of 
animals 
died. 
Mg. 
2 
5 
0 
10 
6 
5 
20 
3 
■ 3 
He also (8) (1902) notes that Gluzihski in 1895 called attention 
to the fact that intravenous injections were more toxic than sub- 
' cutaneous ones. Battelli experimented with what he considered a 
very pure product, using adrenalin base dissolved in water acidulated 
with hydrochloric acid and neutralized with NagCOg just before inject- 
ing into the femoral vein, and obtained the following results : 
Rabbits — 0.1 mg. per kilo, not lethal. 
0.2 mg. per kilo, 1 out of 5 died. 
0.4 mg. per kilo, 3 out of 4 died. 
0.6 mg. per kilo, always lethal. 
Guinea pigs — 0.05 mg. per kilo, not lethal. 
0.10 mg. per kilo, 2 out of 5 died. 
0.20 mg. per kilo, always lethal. 
The toxic dose for the rabbit and guinea pig was about the same 
when the injection was made into the jugular vein of the rabbit 
