14 
and into the femoral vein of the guinea pig, death ensuing from 
oedema of the lungs or fibrillation of the heart. Summing up his 
work, he concludes that intravenous injections are about forty times 
as potent as subcutaneous ones. 
Eeckhout (26) is quoted as finding the lethal dose of adrenalin to 
be 0.08 to 0.06 mg. per kilo. In the original paper, however, I find 
that this lethal dose is calculated from doses that accidentally caused 
death in animals previously injected vdth morphine and atropine 
and anaesthetized vdth chloroform, wliich, according to later writers, 
renders the animals less resistant to adrenahn. 
Amberg (5 and 6) (1902) compared the toxicity of Abel’s epine- 
phrin with the commercial product made by Takamine’s method, and 
found that Abel’s sample dissolved completely, whereas only 517.7 
mg. of the commercial product dissolved in 18 c. c. of HgO, leaving 
behind 4.6 mg. of sediment. After studying the effect of subcu- 
taneous injections upon 9 dogs and intravenous injections upon 16, 
he concluded that the lethal intravenous dose lay between 0.99 and 
2 mg. per kilo, subcutaneous injections of 4.9 mg. per kilo not proving 
lethal, though 6 mg. or more per kilo were. 
Lesage (47, 48, and 49) (1904) does not state by what process his 
adrenalin was made, but judging from the size of the lethal dose it 
must have been a very good one. From a stock solution containing 
0.04 gm. adrenahn, 40 gm. H 2 O, and 1 drop of HCl he made a 1 : 20,000 
solution to be used for intravenous injection. 
Rabbits — 0.05 mg. per kilo, signs of intoxication. 
0.20 mg. per kilo, lethal (4 animals). 
Dogs — 0.05 mg. per kilo, not toxic. 
0.12 mg. per kilo, sometimes lethal. 
0.20-0.25 mg. per kilo, usually lethal (4 out of 6). 
Cats — 0.50-0.81 mg. per kilo, lethal (6 animals survived after 5 injections of 0.25 
mg. and 1 of 0.50 mg. per kilo). 
With the larger doses, 0.26 mg. per kilo, he observed that the dogs 
usually died from asphyxia, but when 0.20 mg. per kilo was fatal ^ 
they usually died from heart failure. In general, anesthetics aug- 
mented the toxic action and sublethal doses of adrenalin rendered 
the animals resistant to doses that ordinarily proved fatal for normal 
animals. He concludes that there is a considerable variation in the 
susceptibility of one individual as compared with another, and a still 
greater variation for different species. 
Baylac, J. (10) (1905), working with a 1 : 1,000 solution of adrenalin, 
found that the lethal dose varies with the species and accordmg to 
the manner of its injection. The lethal subcutaneous dose as deter- 
mined on 6 guinea pigs and 6 rabbits is estimated at 100 mg. per kilo 
and 20 mg. per kilo, respectively. On the other hand^ he estimated 
