36 
catheter; it was always warmed to the body temperature and diluted 
^dth water to about 25 per cent. The same food (cabbage and car- 
rots) was given throughout the entire experiment; no attempt was 
made to determine the amount eaten. The urine was collected tvdce 
a day from a dish placed under the cage; the amount collected in 
twenty-four hours varied considerably. The sulphur determinations 
were made by Folin’s method,® Gooch crucibles being used. Dupli- 
cate analyses were always made. 
Experdiext I. — Rabbit, dark gray; alcohol daily after January 15. 
Date. 
Weight 
in grams. 
Alcohol. 
Total 
S as SO3 
in miUi- 
grams. 
Total 
sulphates 
as SO3 
in milli- 
grams. 
Inorganic 
sulphates 
as SO3 
in milli- 
grams. 
Ethereal 
sulphates 
as SO3 
in milli- 
grams. 
Percent- 
age of 
sulphates 
as ethe- 
real sul- 
phates. 
In grams. 
Grams 
per kilo. 
1905. 
Dec. 14 
Dec. 21 
2,400 
2, 400 
o orkA 
Dec. 25 2, 390 
1906. 
Jan. 2 2,320 
Jan. 8-9 442.5 a 279.2| 269.9 9.3 3.3 
Jan. 9-11 b 266.9 : 258.8 8.1 3.0 
Jan. 15 2,270 6.81 3 ' 
Jan. 21-22 59.7 1 34.0 25.7 43.0 
Jan. 22-23 89.1! 64.9 24.2 27.0 
Jan. 24 2,070 i 
Jan. 26 2,040 i 
Feb. 7-8 189.7 j 175.9! 13.8 7.3 
Feb. 8-9 115.0 1 106.0; 9.0' 7.8 
Feb. 9 2,090 ' 
a 64.5 per cent of total S. 6 Mean of two days. 
Thus, vdthin a week after beginning the alcohol the absolute 
amount of the total sulphate had decreased markedly, but the ethereal 
sulphates had increased from 8 or 9 milligrams to about 25 milli- 
grams; the percentage of sulphate excreted as ethereal sulphate had 
increased from about 3 to 43. The same dose of alcohol (3 grams 
per kilo) was continued daily (vdth the occasional intermission of a 
day) for three weeks; both the absolute and relative amount of 
ethereal sulphate fell. This effect may have been due to the estab- 
lishment of tolerance for the alcohol. 
« Jour. Biol. Cbem., v. 1, p. 131; 1906. In working with rabbits’ urine I have found 
it necessary to frequently renew the asbestos mats of the Gooch crucibles; otherwise 
they either allowed a little barium sulphate to pass through or the filtration, even with 
strong pressure, became extremely slow. I have found the method to work admirably 
with normal human and dog’s urine. 
