11 
Lactarius piperatus. 
Antiseptic. 
Color with guaiacum. 
Color with aloiu. 
Toluene 
Dark blue 
Wine red. 
Chloroform 
None; light blue after long standing. 
Faint pink. 
Fungus No. 7. 
Antiseptic. 
Color with guaiacum. 
Color with aloin. 
Toluene 
Dark blue 
Light red. 
None. 
Do. 
Chloroform 
Thymol 
Very light blue after standing 
Faint blue after long standing 
It is evident, therefore, that the oxidases of certain fungi are less 
readily destroyed toluene than by chloroform or thymol. 
.With the material collected on August IT, 1901, I have been able 
to make out the following additional properties of the oxidases of 
Lepiota americana : 
PRECIPITATION OF THE OXIDASE OF LEPIOTA AMERICANA FROM ITS 
AQUEOUS SOLUTION BY MEANS OF ETHYL ALCOHOL. 
Among other things, the attempt was made to obtain the oxidases 
of Lepiota americana in purer condition by precipitation with ethyl 
alcohol. Accordingly, to some of the perfectly clear 10 per cent 
aqueous extract of the fresh fungus there was added live or six times 
its volume of 95 per cent alcohol. The mixture soon became turbid, 
and in five or six minutes a brown flocculent precipitate made its 
appearance. This was allowed to settle and the liquid above it removed 
by means of a siphon as completely as possible. The remainder was 
then filtered and washed with 95 per cent alcohol. The residue was 
found to consist of a dark-brown colloidal material closely adhering 
to the filter paper. On drying in air it gave a black, shining deposit 
on the paper, which could be removed in the form of scales. This 
was found to be soluble in water, giving rise to a brownish-} T ellow 
solution, which on mixing with the tincture of guaiacum gave a 
splendid dark-blue color at once. 
We see, therefore, that like many other ferments the oxidases of 
Lepiota americana are precipitable from their aqueous solution by 
alcohol in the form of a colloidal precipitate soluble in water. Some 
of this air-dried material was kept in the laboratory in the open air 
for four and one-half months. At the end of this time a small amount 
of it was rubbed up in a porcelain mortar with a few cubic centimeters 
of water; a reddish-brown solution was obtained, which gave a dark 
