25 
der was found to be nearl} T empty. It was removed, however, and put 
in a small amount of water. To some of the solution of the bladder 
contents thus obtained caustic soda was added. The alkaline solution 
thus obtained was practically colorless, or very faintly yellow by 
transmitted light, and by reflected light exhibited a slight greenish 
fluorescence. 
A second portion of the solution of the bladder contents was boiled 
with dilute hydrochloric acid, then cooled and made alkaline with 
caustic soda. The solution thus obtained was decidedly orange yellow 
by transmitted light, and b}^ reflected light exhibited a very marked 
greenish fluorescence. The liver of the pig was removed and washed 
several times with physiological salt solution. It was then preserved 
in normal salt solution by means of toluene over night. The next day 
it was examined in the following manner: 
1. 0.1 gram of the liver was ground to a paste in a porcelain 
mortar and boiled with 10 cc. of water, and made up to 25 cc. This 
mixture was then filtered. 
2. 0.1 gram of the liver was ground to a paste and boiled with 5 cc. 
of water and 5 cc. of dilute hydrochloric acid. This was then made 
up to 25 cc. and filtered. 
Filtrates of (1) and (2) were compared as to fluorescence, b} r adding 
sodium hydroxide to equal amounts of the two filtrates; (1) was found 
to be only faintly fluorescent, whereas (2) was found to be strongly 
fluorescent and b} T transmitted light showed a deeper orange-yellow 
color than (1). 
Ten cc. of filtrate (1) was- boiled with a small amount of dilute 
hydrochloric acid, evaporated somewhat, and then cooled and made 
up to a total volume of 10 cc. Sodium hydroxide was then added to 
this solution and also to 10 cc. of (1). The former showed a decidedh 7 
greater fluorescence than (1). 
It would seem, therefore, that while fluorescein is more rapidly 
absorbed and more toxic than phenolphthalein, it conducts itself in the 
animal organism in much the same manner as the latter compound, 
in that it probabty combines with some substance in the cell, forming 
as the result of this union a compound which is hydrolyzable by 
hydrochloric acid. 
O-CRESOLSULPHONPHTHALEIN. 
On October 20, 1905, at 10.15 a. m., 0.0761 gram of o-cresolsul- 
■ phonphthalein was mixed with water and the mixture injected into the 
peritoneal cavity of a guinea pig weighing 100 grams. At 10.20 a. m., 
same day, the animal passed urine which gave no coloration either 
with sodium lydroxide alone or after boiling with lydrochloric acid. 
At 1.30 p. m., same date, the animal passed urine having a deep pur- 
plish-red color. With hydrochloric acid this changed to a dark 
19948— No. 26—06 4 
