29 
the animal organism, and } 7 et, as was proved in connection with these 
experiments, the substances present in the liver in no way interfere 
with the hydrolysis of the compound by hot hydrochloric acid. 
The action of the saliva on the phenolphtbalein compound of the 
urine was also tried. Two cc. of the fresh urine containing the phe- 
nolphthalein compound were mixed with 5 cc. of human saliva and a 
small amount of toluene added as a preservative. This mixture was 
then allowed to stand for 24 hours. At the end of this time, sodium 
hydroxide was then added in slight excess, when the solution took 
on a decided but faint pink coloration. It would seem, therefore, that 
saliva has the power of l^drolyzing the compound of phenolphthalein, 
at least to a slight extent. It is my intention to continue this inves- 
tigation, in the hope of being able to obtain this phenolphthalein 
compound in a condition of purity, with the view of determining its 
composition and of more carefully studying its conduct toward acids, • 
micro-organisms, and ferments. 
