48 
The following are the conditions and 
periments. 
EXPERIMENT NO. 
results of the different ex- 
23. 
Autoclave. 
Formalin, 600 c. c.; glycerin, 60 c. c.; calcium chloride, 120 grams; water sufficient to make 
1,000 c. c. 
Capacity of room, 2,000 cubic feet. 
Quantity of formaldehyde used per cubic foot, 0.107 gram. 
Time required to exhaust material in autoclave, 25 minutes. 
Quantity formaldehyde found per cubic foot, 0.04213 gram. 
(Determination made by drawing air from room beginning 35 minutes after exhausting auto- 
clave and consuming 1 hour 17 minutes in the drawing.) 
Yield of formaldehyde, 39.37 per cent. 
Weather: Moderate westerly wind; cloudy. 
Tempera- 
ture. 
Relative 
humidity. 
Absolute 
humidity 
(grams per 
cubic foot). 
Before beginning to introduce the gas 
° F. 
81.5 
Per cent. 
81 
9. 26 
After 30 minutes 
83. 0 
94 
11. 26 
After 2 hours 
» 
82.0 
86 
10. 00 
Note.— I n all the experiments with this method the temperature and humidity are given from the 
time of beginning to introduce the gas; the organisms were exposed immediately after exhausting the 
autoclave. 
[+ means growth; — no growth.] 
B.coli communis .* 
B. pyocyaneus . . . 
B. typhosus 
B. coli communis. 
B. dysenteric 
B. subtilis 
B. coli communis . 
Organism. 
How exposed. 
Glass 
Filter paper. . . 
do 
do 
do 
do 
Wilson method 
Time of exposure in 
minutes, and results. 
2 
5 
10 
20 
30 
60 
+ 
— 
— 
— • 

— 
+ 
+ 
- 
- 
- 
- 
+ 
- 
— 
— 
— 
— 
+ 
+ 
- 
- 
— 
+ 
+ 
— 
— 
— 
— 
+ 
+ 
— 
- 
— 
— 
EXPERIMENT NO. 24. 
Autoclave. 
Formalin, 600 c. c.; glycerin, 60 c. c.; calcium chloride, 120 grams; water sufficient to make 
1,000 c. c. 
Capacity of room, 2,000 cubic feet. 
Quantity of formaldehyde used per cubic foot, 0.107 gram. 
Time required to exhaust material in autoclave, 25 minutes. 
Quantity formaldehyde found per cubic foot, 0.0406 gram. 
(Determination made by drawing air from room beginning 3 hours 32 minutes after exhausting 
autoclave and consuming 1 hour 18 minutes in the drawing.) 
Yield of formaldehyde, 37.94 per cent. 
Weather: Practically no wind; cloudy. 
Tempera- 
ture. 
Relative 
humidity. 
Absolute 
humidity 
(grams per 
cubic foot). 
Before beginning to introduce the gas 
°F. 
80 
82 
81 
Per cent. 
75 
96 
91 
8. 20 
11. 16 
10. 58 
After 30 minutes 
After 2 hours _ 
See Note, Experiment No. 23. 
