49 
EXPERIMENT NO. 24— Continued. 
EXPERIMENT NO. 25. 
Autoclave. 
Formalin, 600 c. c.; glycerin, 60 c. c.; calcium chloride, 120 grams; water sufficient to make 1,000- 
c. c. 
Capacity of room, 2,000 cubic feet. 
Quantity of formaldehyde used per cubic foot, 0.107 gram. 
Time required to exhaust material in autoclave, 22 minutes. 
Quantity formaldehyde found per cubic foot, 0.04448 gram. 
(Determination made by drawing air from room, beginning 40 minutes after exhausting auto- 
clave, and consuming 1 hour 10 minutes in the drawing.) 
Yield of formaldehyde, 41.57 per cent. 
Weather: Slight westerly breeze. 
Tempera- 
ture. 
Relative 
humidity. 
Absolute 
humidity 
(grams per 
cubic foot). 
Before beginning to introduce the gas 
° F. 
80.0 
80.5 
81.0 
Per cent. 
75 
94 
94 
8. 20 
10. 43 
10. 59 
After 30 minutes 
After 2 hours 
See Note, experiment No. 23. 
[+ means growth; — no growth.] 
The above experiments show very efficient germicidal results. Even 
the spores of B. subtilis exposed by the Wilson method were killed 
within 10 minutes in experiments Nos. 23 and 24. 
In experiment No. 25 the time required to kill the different organ- 
isms was prolonged as compared with the two previous experiments; 
