51 
EXPERIMENT NO. 27. 
Autoclave. 
Formalin, 600 c. c.; glycerin, 60 c. c. ; calcium chloride, 120 grams; water sufficient to make 1,000 
c. c. 
Capacity of room, 2,000 cubic feet. 
Quantity of formaldehyde used per cubic foot, 0.107 gram. 
Time required to exhaust material in autoclave, 25 minutes. 
Quantity formaldehyde found per cubic foot, 0.01851 gram. 
(Determination made by drawing air from room, beginning 15 minutes after exhausting auto- 
clave, and consuming 1 hour 7 minutes in the drawing.) 
Yield of formaldehyde, 17.30 per cent. 
Weather: Very little wind. 
Tempera- 
ture. 
Relative 
humidity. 
Absolute 
humidity 
(grams per 
cubic footl. 
Before beginning to introduce the gas 
° F. 
49 
Per cent. 
67 
2.63 
After 30 minutes 
51 
81 
3.41 
After 2 hours 
54 
61 
2.85 
See Note, experiment No. 23. 
[+ means growth; — no growth.] 
Organism. 
How exposed. 
B. coli communis 
B. typhosus 
B. subtilis 
B. coli communis 
B. subtilis 
Filter paper . . . 
do 
do 
Wilson method 
do 
Time of exposure in minutes, 
and results. 
10 
20 
30 
45 
60 
120 
180 
+ 
+ 
+ 
+ 
+ 
+ 
— 
— 
+ 
+ 
+ 
+ 
+ 
— 
— 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
EXPERIMENT NO. 27. 
Autoclave. 
Formalin 600 c. c., glycerin 60 c. c., calcium chloride 120 grams, water sufficient to make 1,000 c. c. 
Capacity of room, 2,000 cubic feet. 
Quantity of formaldehyde used per cubic foot, 0.107 gram. 
Time required to exhaust material in autoclave, 28 minutes. 
Quantity formaldehyde found per cubic foot, 0.01471 gram. 
(Determination made by drawing air from room, beginning 15 minutes after exhausting auto- 
clave and consuming 1 hour 30 minutes in the drawing.) 
Yield of formaldehyde, 13.75 per cent. 
Weather: Damp and snow on ground; light wind. 
Tempera- 
ture. 
Relative 
humidity. 
Absolute 
humidity 
(grams per 
cubic foot) . 
Before beginning to introduce the gas 
O J? 
41 
Per cent. 
69 
2.04 
After 30 minutes 
42 
92 
2.81 
After 2 hours 
42 
84 
2.57 
See Note, Experiment No. 23. 
