79 
EXPERIMENT No. 41. 
Formalin 2,000 c. c., permanganate 1,000 grams, equally distributed in four galvanized-iron buck- 
ets, two of which were placed in the main sleeping compartment of car and one in the alleyway 
at each end. 
Weather: Clear; light southerly wind. 
Temperature in car at beginning of experiment, 83° F. 
Relative humidity in car at beginning of experiment, 78 per cent. 
Time of exposure, 2 hours (11.15 a. m. to 1.15 p. m.). 
Temperature in car after opening and airing 15 minutes, 87° F. 
Relative humidity in car after opening and airing 15 minutes, 62 per cent. 
[+ means growth; — no growth.] 
Organism. 
Place of exposure in car. 
Result. 
B. coli communis 
Toilet room 
Smoking room 
Drawing-room 
— 
Floor main sleeping compartment 
— 
Berth, lower No. 6 
— 
B. diphtherias 
Smoking room 
Drawing-room 
— 
Floor main sleeping compartment 
— 
Berth, upper No. 4 
— 
/ 
Berth, lower No. 8 
— 
B. typhosus 
Toilet room 
Smoking room 
— 
Drawing-room 
— 
Floor main sleeping compartment 
— 
Berth, upper No. 6 
— 
B. subtilis 
Smoking room 
Drawing-room 
— 
Berth, upper No. 10 
— 
Berth, lower No. 8 
— 
B. tuberculosis 
Smoking room 
Sputum exposed and injected into guinea pig; pig chloro- 
formed 100th day; increased 255 grams in weight; no lesions of 
tuberculosis. 
Floor of main sleeping compartment 
— 
Sputum exposed and injected into guinea pig; pig chloro- 
formed 100th day; increased 235 grams in weight; no lesion of 
tuberculosis. 
Control pig died of tuberculosis 45th day. 
32507 — No. 27—06 6 
