81 
EXPERIMENT NO. 43. 
Formalin 2,000 c. c., permanganate 1,000 grams, equally distributed in four galvanized-iron buck- 
ets, two of which were placed in the main sleeping compartment of car and one in. the alleyway 
at each end. 
Weather: Clear and dry, with practically no wind. 
Temperature in car at beginning of experiment, 77.5° F. 
Relative humidity in car at beginning of experiment, 69 per cent. 
Time of exposure, 2 hours (11.45 a. m. to 1.45 p. m.). 
Temperature in car after opening and airing 15 minutes, 83° F. 
Relative humidity in car after opening and airing 15 minutes, 53 per cent. 
[+ means growth; — no growth.] 
Organism. 
Place of exposure in car. 
B. coli communis 
B. diphtheria 
B. pyocyaneus 
B. subtilis 
Toilet room 
Smoking room 
Drawing-room 
Berth, upper No. 9 
Berth, lower No. 8 
Toilet room 
Smoking room 
Drawing-room 
Floor main sleeping compartment 
Berth, lower No. 6 
Toilet room 
Smoking room 
Drawing-room 
Toilet room 
Smoking room 
Drawing-room 
Berth, lower No. 2 
Result. 
+ 
+ 
