83 
EXPERIMENT NO. 45. 
Formalin, 2,000 c. c.; permanganate, 1,000 grams, equally distributed in four galvanized-iron 
buckets, two of which were placed in the main sleeping compartment of car and one in the alley 
way at each end. 
Weather: Clear and dry, with light variable wind. 
Temperature in car at beginning of experiment, 74° F. 
Relative humidity in car at beginning of experiment, 65 per cent. 
Time of exposure, 2 hours (11.30 a. m. to 1. 30 p. m.). 
Temperature in car after opening and airing 15 minutes, 82° F. 
Relative humidity in car after opening and airing 15 minutes, 58 per cent. 
[-1- means growth; — no growth.] 
Organism. 
Place of exposure in car. 
Result. 
R coli communis 
Toilet room 
Smoking room 
Drawing-room 
Floor, main sleeping compartment 
+ 
Berth, lower No. 10 
B. diphtherias 
Toilet room 
Smoking room 
Drawing-room 
Floor, main sleeping compartment 
Berth, upper No. 8 
B. typhosus 
Toilet room 
Smoking room 
Drawing-room 
Berth, upper No. 4 
Berth, upper No. 10 
R. subtilis 
Smoking room 
Toilet room 
D rawing-room 
+ 
Berth, upper No. 8 
Berth, upper No. 4 
+ 
R. tuberculosis 
Smoking room 
Sputum exposed and injected into guinea pig. Pig chloro- 
formed 105th day; increased 325 grams in weight; no lesions of 
tuberculosis. 
Floor of main sleeping compartment 
\ 
Sputum exposed and injected into guinea pig; pig chloroformed 
105th day; increased 245 grams in weight; no lesion of tuberculosis. 
Drawing-room 
Sputum exposed and injected into guinea pig; pig chloro- 
formed 105th day; increased 270 grams in weight; no lesions of 
tuberculosis. 
Control guinea pig died of tuberculosis 45th day. 
