85 
In the following experiments (Nos. 47 to 54, inclusive) it will be seen 
that when the temperature is relatively cold or the humidity compara- 
tively low the formaldehyde gas fails to kill many of the organisms 
exposed. 
It is especially interesting to note in the following experiments that 
while the results can not be considered satisfactory from a practical 
standpoint, because many of the organisms exposed escaped destruc- 
tion, nevertheless the interesting point is brought out that organisms 
exposed in the toilet room of the sleeping car were almost invariably 
killed. No doubt the reason for this is the greater amount of moisture 
in the toilet room owing to the water present. As the toilet room is 
probably more apt to be infected than any other part of the coach, 
this is a matter of considerable practical value. 
EXPERIMENT NO. 47. 
Formalin 1,500 c. c., permanganate 750 grams, equally distributed in three iron buckets, one of 
which was placed in the main sleeping compartment of car and one in the alleyway at each end. 
Weather: Clear, light variable wind. 
Temperature in car at beginning of experiment, 78° F. 
Relative humidity in car at beginning of experiment 63 per cent. 
Time of exposure, 2 hours (12 m. to 2 p. m.). 
Temperature in car after opening and airing 15 minutes, 83° F. 
Relative humidity in car after opening and airing 15 minutes, 58 per cent. 
[+ means growth; — no growth.] 
Organism. 
B. coli communis 
B. typhosus 
B. pyocyaneus 
Staphyllococcus pyo 
genes aureus. 
B. subtilis 
Place of exposure in car. 
Toilet room 
Smoking room 
Drawing-room 
Berth, upper No. 3 
Berth, lower No. 6 
Toilet room 
Smoking room 
Drawing-room 
Floor main sleeping compartment 
Berth, upper No. 12 
Berth, lower No. 11 
Berth, lower No. 3 
Toilet room 
Smoking room 
Drawing-room 
Floor main sleeping compartment 
Berth, upper No. 3 
Berth, upper No. 8 
Berth, lower No. 4 
Berth, lower No. 11 
Toilet room 
Smoking room 
Berth, upper No. 6 
Berth, upper No. 12 
Berth, lower No. 3 
Smoking room 
Berth, upper No. 11 
Berth, lower No. 3 
Result. 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
