90 
EXPERIMENT NO. 52. 
Formalin 2,000 c. c., permanganate 1,000 grams, equally distributed in four galvanized-iron buck- 
ets, two of which were placed in the main sleeping compartment of car and one in the alleyway 
at each end. 
Weather: Clear, rather strong northerly wind. 
Temperature in car at beginning of experiment, 70° F. 
Relative humidity in car at beginning of experiment. 48 per cent. 
Time of exposure, 2 hours (11.25 a. m. to 1.25 p. m.). 
Temperature in car after opening and airing 15 minutes, 72° F. 
Relative humidity in car after opening and airing 15 minutes, 45 per cent. 
[+ means growth; — no growth.] 
Organism. 
Place of exposure in car. 
Result. 
B. coli communis 
Toilet room 
Smoking room 
Floor main sleeping compartment 
+ 
Berth, upper No. 10 
— 
Berth, lower No. 9 
B. diphtheria 
Toilet room 
Smoking room 
Drawing room 
-f- 
Floor main sleeping compartment 
+ 
Berth, upper No. 8 
- 
B. ty'phosi'rS 
Toilet room 
__ 
Smoking room 
— 
Floor main sleeping compartment 
+ 
Berth, upper No. 4 
- 
Berth, lower No. 10 
+ 
B. subtilis 
Smoking room 
+ 
Drawing room 
4 - 
Floor main sleeping compartment 
+ 
Berth, upper No. 2 
+ 
Berth, lower No. 6 : 
+ 
B. tuberculosis 
Floor of main sleeping compartment 
+ 
Sputum exposed and injected, into guinea pig; pig chloro- 
formed 87th day; increased 125 grams in weight; tuberculous 
lesions in liver, spleen, lymph glands, etc. 
Floor of main sleeping compartment 
•4 
Sputum exposed on carpet and injected into guinea pig; pig 
chloroformed 87th day: increased 40 grams in weight; marked 
tuberculous lesions in liver, spleen, glands, etc. 
Control guinea pig died of tuberculosis 50th day. 
• - 
