91 
EXPERIMENT NO. 53. 
Formalin 2,000 c. c., permanganate 1,000 grams, equally distributed in four galvanized-iron buck- 
ets, two of which were placed in the main sleeping compartment of car and one in the alleyway 
at each end. 
Weather: Clear, practically no wind. 
Temperature in car at beginning of experiment, 65° F. 
Relative humidity in car at beginning of experiment, 44 per cent. 
Time of exposure, 2 hours (8.25 a. m. to 10.25 a. m.). 
Temperature in car after opening and airing 15 minutes, 69° F. 
Relative humidity in car after opening and airing 15 minutes, 55 per cent. 
[4- means growth; — no growth.] 
Organism. 
Place of exposure in car. 
Result. 
B . coli communis 
Smoking room 
+ 
Drawing room 
Floor main sleeping compartment 
Berth, lower No. 8 
4 - 
Berth, lower No. 4 
B. diphtherias 
Drawing room 
+ 
Smoking room 
+ 
Floor main sleeping compartment 
+ 
Berth, upper No. 6 
4 - 
Berth, lower No. 10 
4- 
B. typhosus 
Drawing room 
4- 
Smoking room 
4 - 
Floor main sleeping compartment 
4- 
Berth, upper No. 10 
Berth, lower No. 10 
4 - 
R sv ht.ilis 
Toilet room 
4- 
Smoking room 
4 - 
Floor main sleeping compartment 
4 - 
Berth, upper No. 7 
4- 
' 
Berth, lower No. 4 
4 - 
B . tuberculosis 
Floor of main sleeping compartment 
4 - 
' 
■ . . 
Sputum exposed and injected into guinea pig; pig chloro- 
formed 86th day; increased 35 grams in weight; tuberculous 
lesions in liver, spleen. 
Floor of main sleeping compartment 
+ 
Sputum exposed on carpet and injected into guinea pig; pig 
chloroformed 86th day; increased 70 grams in weight; tuber- 
culous lesions in spleen, mesentery, lymph glands. 
Control guinea pig died of tuberculosis 50th day. 
The above experiment was done in the morning (8.25 to 10.25), at 
which time the relative humidity is high; but as the car had been 
artificially heated the night before the humidity was low. This prob- 
ably accounts for the inefficient results obtained. 
In the following experiment, temperature 46° F., polymerization 
was so marked that the deposit of paraform gave the interior furnish- 
ings of the car a frosty or white appearance. 
