93 
EXPERIMENT NO. 55. 
Formalin, 2,000 c. c., permanganate, 1,000 grams, equally distributed in four galvanized-iron 
buckets, two of which were placed in main sleeping compartment and one in the alleyway 
at each end. 
Weather: Clear, moon shining, very little breeze. 
Temperature in car at beginning of experiment, 77° F. 
Relative humidity in car at beginning of experiment, 69 per cent. 
Time of exposure, 2 hours (8 p. m. to 10 p. m.). 
Temperature in car after opening and airing 15 minutes. 72° F. 
Relative humidity in car after opening and airing 15 minutes, 71 per cent. 
Official relative humidity at 2 p. m. of the date on which the experiment was done, 26 per cent. 
[+ means growth; — no growth.] 
Organism. 
Place of exposure in car. 
Result. 
B. coli communis 
Drawing-room 

Smoking room 
— 
Floor main sleeping compartment 
- 
Berth, upper No. 7 
- 
Berth, lower No. 6 
— 
B. diphtherias 
Toilet room 

Smoking room 
Floor main sleeping compartment 
— 
Berth, lower No. 10 
- 
B. typhosus 
Toilet room 
_ 
Smoking room 
Floor main sleeping compartment 
— 
Berth, lower No. 8 
— 
Berth, upper No. 11 
- 
B. subtilis 
Toilet room 

Smoking room 
Floor main sleeping compartment 
-I- 
Berth, upper No. 8 
+ 
Berth, lower No. 9 
+ 
B. tuberculosis 
Floor main sleeping compartment 
-I- 
Sputum exposed by Wilson method and injected into guinea 
pig; pig chloroformed 88th day; increased 115 grams in weight; 
lesions of tuberculosis in spleen, liver, lungs, and lymph glands. 
Floor main sleeping compartment 
+ 
Sputum exposed on carpet and injected into guinea pig; pig 
chloroformed SSth day; increased but 5 grains in weight; lesions 
of tuberculosis In liver and spleen. 
Control guinea pig died of tuberculosis 63d day. 
