94 
EXPERIMENT NO. 56. 
Formalin 2,000 c. c., permanganate 1,000 grams, equally distributed in four galvanized-iron 
buckets, two of which were placed in main sleeping compartment and one in the alleyway at 
each end. 
Weather: Clear, moon shining, no wind. 
Temperature in car at beginning of experiment, 61.5° F. 
Relative humidity in car at beginning of experiment, 64 per cent. 
Time of exposure, 2 hours (8.35 p. m. to 10.35 p. m.). 
Temperature in car after opening and airing 15 minutes, 57° F. 
Relative humidity in car after opening and airing 15 minutes, 66 per cent. 
Official relative humidity at 2 p. m. of the date on which the experiment was done, 39 per cent. 
[+ means growth; — no growth.] 
Organism. 
Place of exposure in car. 
Result. 
B. coli communis 
Toilet room 
- 
Smoking room 
Floor main sleeping compartment 
Berth, upper No. 4 
Berth, lower No. 9 
-1- 
B. diphtherias 
D ra wing-ro om 
Smoking room 
Floor main sleeping compartment 
Berth, lower No. 10 
Berth, upper No. 8 
B. typhosus 
Toilet room 
Smoking room. .* 
D ra wing-room 
Floor main sleeping compartment 
Berth, lower No. 6 
B. suhtilis 
D rawing-room 
+ 
Smoking room 
Floor main sleeping compartment 
Berth, upper No. 5 ' 
+ 
Berth, lower No. 4 
+ 
B. tuberculosis 
Floor main sleeping compartment 
Sputum exposed by Wilson method and injected into guinea 
pig; pig chloroformed 63d day; increased 270 grams in weight; 
no lesions of tuberculosis. 
Finer main sleeping compartment 
+ 
Sputum exposed on carpet and injected into guinea pig; pig 
chloroformed 63d day; increased 150 grams in weight; lesions of 
tuberculosis in liver, spleen, and lymph glands. 
Control guinea pig died of tuberculosis 37th day. 
