95 
EXPERIMENT NO. 57. 
Formalin 1,500 c. c., permanganate 750 grams, equally distributed in three iron buckets, one 
of which was placed in the main sleeping compartment of car and one in the alleyway at each end. 
Weather: Clear and moonshiny, no wind. 
Temperature in car at beginning of experiment, 59° F. 
Relative humidity in car ax beginning of experiment, 57 per cent. 
Time of exposure, 2 horns (8.40 p. m. to 10.40 p. m.). 
Temperature in car after opening and airing 20 minutes, 55° F. 
Relative humidity in car after opening and airing 20 minutes, 62 per cent. 
Official relative humidity at 2 p. m. of the date on which the experiment was performed, 39 per cent. 
[+ means growth; — no growth.] 
Organism. 
Place of exposure in car. 
Result. 
B. coli communis 
Toilet room 
Smoking room 
Floor main sleeping compartment 
-1- 
Berth, lower No. 6 
B. diphtherix 
Toilet room 
Smoking room 
+ 
Floor main sleeping compartment 
Berth, upper No. 5 
Berth, lower No. 10 
B. typhosus 
Smoking room 
Drawing-room ' 
+ 
Floor main sleeping compartment 
+ 
Berth, upper No. 10 
Berth, lower No. 4 
+ 
B. subtilis 
Toilet room 
+ 
Smoking room 
4- 
D ra wing-room 
+ 
Floor main sleeping compartment 
+ 
Berth, upper No. 4 
+ 
B. tuberculosis 
Floor main sleeping compartment 
Sputum exposed by the Wilson method and injected into 
guinea pig; pig chloroformed 63d day; increased 370 grams in 
weight; no lesions of tuberculosis. 
Floor main sleeping compartment 
+ 
Sputum exposed on carpet and injected into guinea pig; pig 
chloroformed 63d day; increased 160 grams in weight; spleen, 
lungs, and lymph glands tuberculous. 
Control guinea pig died of tuberculosis 37th day. 
32507 — No. 27 
06 7 
