96 
U. DILUTED FOKAI A LiN-I’KR'VIAX G AX V 1 E 3EETHOD. 
The following four experiments were done with smaller quantities 
of formalin diluted with varying quantities of water before mixing 
with the permanganate. All except the first were done on compara- 
tively dry days, with the object of determining whether the addition 
of the water would sufficiently increase the humidity to obtain effi- 
cient results on such days, as it has been satisfactorily demonstrated 
in previous experiments that moisture is one of the most essential 
factors in formaldehyde disinfection: 
EXPERIMENT NO. 58. 
Formalin 1,000 c. c., water 1,000 c. c.. permanganate 1,000 grams, equally distributed in four galvan- 
ized-iron buckets, two of which were placed in the main sleeping compartment of car and one in 
the alleyway at each end. 
Weather: Clear, light southerly wind. 
Temperature in car at beginning of experiment, 85° E. 
Relative humidity in car at beginning of experiment, 73 per cent. 
Time of exposure, 2 hours (11.30 a. m. to 1.30 p. m.). 
Temperature in car after opening and airing 15 minutes, 88° F. 
Relative humidity in car after opening and airing 15 minutes, 54 per cent. 
[4- means growth, — no growth.] 
Organism. 
Place of exposure in car. 
Result. 
B . coli communis 
Toilet room . . . , 

Smoking room 
- 
Drawing-room 
- 
Floor main sleeping compartment 
- 
Berth, lower No. 6 
— 
B. diphtherise 
Smoking room 
— 
Drawing-room 
- 
Floor main sleeping compartment 
■ 
Berth, upper No. 6 
- 
Berth, lower No. 8 
- 
B. typhosus 
Toilet room 
- 
Smoking room 
- 
Drawing-room 
- 
Floor main sleeping compartment 
- 
Berth upper No. 10 
- 
B. suhtilis 
Smoking room 
+ 
Drawing-room - 
T 
Floor main sleeping compartment 
+ 
Berth, upper No. 6 
B. tuberculosis 
Smoking room 
+ 
Sputum exposed and injected into guinea pig: pig chloro- 
formed 101st day; increased 200 grams in weight: marked lesions 
of tuberculosis in spleen and lymph glands, slight appearances in 
liver. 
Floor main sleeping compartment 
Sputum exposed and injected into guinea pig; pig chloro- 
— 
formed 101st day; increased 240 grams in weight: no lesions of 
tuberculosis. 
Control pig died of tuberculosis on 45th day. 
■ ■ | 
