97 
EXPERIMENT NO. 59. 
Formalin 1,500 c. c., water 500 c. c., permanganate 1,000 grams, equally distributed in four galvan- 
ized-iron buckets, two of which were placed in the main sleeping compartment of car and one in 
the alleyway at each end. 
Weather: Dry and clear, light southwesterly wind. 
Temperature in car at beginning of experiment, 76° F. 
Relative humidity in car at beginning of experiment, 51 per cent. 
Time of exposure, 2 hours (11.45 a. m. to 1.45 p. nr.). 
Temperature in car after opening and airing 15 minutes, 78° F. 
Relative humidity in car after opening and airing 15 minutes, 36 per cent. 
[4- means growth, — no growth.] 
Organism. 
Place of exposure in cah. 
Result. 
B. coli communis 
Toilet room 

Drawing-room 
- 
Floor main sleeping compartment 
- 
Berth, lower No. 8 
+ 
Berth, upper No. 5 
- 
B. diphtherise 
Toilet room 
- 
. 
Smoking room 
+ 
Floor main sleeping compartment 
+ 
Berth, upper No. 9 
+ 
Berth, lower No. 12 
+ 
B. typhosus 
Toilet room 
_ 
Drawing-room 
Floor main sleeping compartment 
- 
Berth, upper No. 9 
+ 
Berth, lower No. 4 
— 
B. subtilis 
Toilet room 
_ 
Drawing-room 
+ 
Floor main sleeping compartment 
+ 
Berth, lower No. 2 
+ 
B. tuberculosis 
Drawing-room 
_ 
Sputum exposed and injected into guinea pig; pig chloro- 
formed 100th day: increased 270 grams in weight; no lesions of 
tuberculosis. 
Floor main sleeping compartment 
+ 
>> 
Sputum exposed and injected into guinea pig; pig died of gen- 
eral tuberculosis on 88th day. 
Control pig died of tuberculosis on 63d day. 
