99 
EXPERIMENT NO. 61. 
Formalin, 1,500 c. c.; water, 500 c. c.; permanganate, 1,000 grams, equally distributed in four galva- 
nized-iron buckets, two of which were placed in the main sleeping compartment of car and one 
in the alleyway at each end. 
Weather: Clear, southerly wind. 
Temperature in car at beginning of experiment, 79° F. 
Relative humidity in car at beginning of experiment, 66 per cent. 
Time of exposure, 2 hours (12 m. to 2 p. m.). 
Temperature in car after opening and airing 15 minutes, 86° F. 
Relative humidity in car after opening and airing 15 minutes, 53. per cent. 
[4- means growth; — , no growth.] 
Organism. 
Place of exposure in car. 
Result 
B. coli communis 
Toilet room 
Smoking room 
Drawing-room 
Floor main sleeping compartment 
Berth, lower No. 11 
B. diphtherise 
Toilet room 
Smoking room 
+ 
Drawing-room 
+ 
Floor main sleeping compartment - 
Berth, upper No. 8 
+ 
B. typhosus 
Toilet room 
Smoking room 
Floor main sleeping compartment 
Berth, upper No. 10 
Berth, lower No. 6 
B. subtilis 
Smoking room 
Drawing-room 
+ 
Floor main sleeping compartment 
+ 
Berth, upper No. 4 
B. tuberculosis 
Smoking room 
+ 
Sputum exposed and injected into guinea pig; pig chloro- 
formed 100th day; increased 200 grams in weight; lesions of tu- 
berculosis in spleen and lymph glands. 
Floor main sleeping compartment 
+ 
Sputum exposed and injected into guinea pig; pig chloro- 
formed 100th day; marked lesions of tuberculosis in liver and 
spleen. 
Control pig died of tuberculosis on 60th da}". 
The results of the previous experiments done on dry days show that 
the organisms \uere usually not destroyed, and consequently no prac- 
tical advantage gained by adding the water to the formalin. How- 
ever, experiment No. 58 again proves our conclusions, namely, that 
successful disinfection does not depend so much upon the amount of 
gas as upon the temperature and humidity. Although a less quantity 
of formalin was used in this experiment than in the other three, the 
results were very much better. 
III. RETORT WITHOUT PRESSURE. 
A few experiments were done with this method, and as only one 
retort was used in each experiment, it was changed from one end of 
the car to the other at definite intervals during the process of vapor- 
izing the formalin. 
