100 
The locks of the car doors were removed in order to introduce the 
nozzle of the retort. The heat was under the retort 30 to 40 minutes 
before gas began to be given off, and then from 1 hour 30 minutes to 
2 hours, depending upon temperature conditions in the different 
experiments, was required to completely vaporize the formalin. In 
one experiment, temperature 52 c F.. about 300 c. c. of liquid still 
remained in the retort at the end of the experiment. The time of 
exposure of the organisms was counted from the time of beginning 
to introduce the gas. 
The experiments are as follows: 
EXPERIWEXT XO. 62. 
Retort without pressure: formalin 2,000 c. c. 
Time required to vaporize formalin, 1 hour 40 minutes. 
Retort changed from one end of the car to the other every 20 minutes during process of vaporizing 
the formalin. 
W eather : Damp and cloudy, light wind. 
Temperature in car at beginning of experiment. 75 = E. 
Relative humidity in car at beginning of experiment. 71 per cent. 
Time of exposure. 2 hours, from time of beginning to introduce the gas 11 a. m. to 1.00 p. m.) 
Temperature in car after opening and airing 30 minutes, SI : F. 
Relative humidity in car after opening and airing 30 minutes. 6-5 per cent. 
[ — means growth : — . no growth.] 
Organism. 
Place of exposure in car. 
Result. 
B. coli communis 
Smoking room 
— 
Drawing-room 
- 
Floor main sleeping compartment 
— 
Berth, upper Xo. 8 
- 
Berth, lower Xo. 6 
- 
B. diphihcri.se 
Smoking room 
— 
D ra wing-room 
- 
Floor main sleeping compartment 
- 
Berth, upper Xo. 4 
— 
Berth, lower Xo. 10 - 
— 
B. tpvhosus 
Smoking room 
— 
D ra wing-room 
— 
Floor main sleeping compartment 
Berth, upper Xo. 6 
— 
Berth, lower Xo. 4 
- 
B. subtUis 
Toilet room 
— 
Smoking room 
- 
Floor main sleeping compartment 
-r 
Berth, upper Xo. 2 
+ 
Berth, lower Xo. S 
+ 
B. tuberculosis 
Floor main sleeping compartment 
Sputum exposed and injected into gainea pig: pig chloro- 
formed 99th day: increased 190 grams in weight: tuberculous 
lesions in spleen and lymph glands. 
Floor main sleeping compartment 
+ 
Sputum exposed on carpet and injected into guinea pig; pig 
died of tuberculosis on 50th day. 
Control guinea pie died of tuberculosis on 60th day. 
- 
