101 
EXPERIMENT NO. 63. 
9 
Retort without pressure; formalin 2,000 c. c. 
Time required to vaporize formalin 1 hour and 30 minutes. 
Retort changed from one end of car to the other every 15 minutes during process of vaporizing the 
formalin. 
Weather : Dry and clear, light southwesterly wind. 
Temperature in car at beginning of experiment, 73° F. 
Relative humidity in car at beginning of experiment, 57 per cent. 
Time of exposure, 2 hours, from time of beginning to introduce the gas (12 m. to 2 p. m.). 
Temperature in car after opening and airing 15 minutes, 78° F. 
Relative humidity in car after opening and airing 15 minutes, 38 per cent. 
[+means growth; — , no growth.] 
Organism. 
Place of exposure in car. 
Result. 
B. coli communis 
Toilet room 
Drawing room 
Floor, main sleeping compartment 
~ 
Berth, upper No. 4 
i 
B. diphtherias 
Berth, lower No. 8 
Drawing room 
+ 
+ 
Floor, main sleeping compartment 
Berth, upper No. 8 
Berth, upper No. 10 
+ 
+ 
Berth, lower No. 12 
+ 
B. typhosus 
Toilet room 
Drawing room 
Floor, main sleeping compartment 
+ 
Berth, lower No. 7 
B. subtilis , . 
Toilet room . 
Drawing room 
+ 
Floor, main sleeping compartment 
+ 
Berth, upper No. 12 
+ 
Berth, lower No. 8 
+ 
B. tuberculosis 
Drawingroom 
+ 
Sputum exposed and injected into guinea pig; pig chloroformed 
97th day; marked lesions of tuberculosis in spleen. 
Floor, main sleeping compartment 
Sputum exposed and in jected into guinea pig; pig chloroformed 
97th day; increased 300 grams in weight; no lesions of tuberculosis. 
Control guinea pig died of tuberculosis 63d day. 
