103 
During this process the autoclave was changed from one end of the 
car to the other every 10 or 15 minutes. As with the retort, it was 
necessary to remove the locks from the doors in order to introduce the 
nozzle of the autoclave. 
The experiments are as follows : 
EXPERIMENT NO. 65. 
Autoclave, formalin 2 000 c. c. 
Time required to vaporize formalin, 45 minutes. 
Autoclave changed from one end of car to other every 15 minutes during process of vaporizing the 
formalin. 
Weather: Clear; southerly breeze. 
Temperature in car at beginning of experiment, 78° F. 
Relative humidity at beginning of experiment, 67 per cent. 
Time of exposure, 2 hours (11.55 a. m. to 1.55 p. m.) from time of beginning to introduce the gas. 
Temperature in car after opening and airing 30 minutes, 87° F. 
Relative humidity after opening and airing 30 minutes, 50 per cent. 
I [+ means growth; — , no growth]. 
Organism. 
Place of exposure in car. 
Result. 
B. coli communis 
B . diphtherias 
B. typhosus 
B. subtilis 
B . tuberculosus 
Toilet room 
Smoking room. 
Drawing-room 
Floor main sleeping compartment 
Berth, upper No. 8 
Toilet room 
Smoking room .• 
Floor main sleeping compartment 
Berth, upper No. 8 
Berth, lower No. 4 
Toilet room 
Smoking room 
Drawing-room 
Floor main sleeping compartment 
Berth, lower No. 10 
Smoking room 
Drawing-room 
Floor main sleeping compartment 
Berth, upper No. 6 
Smoking room 
Sputum exposed and injected into guinea pig. Pig chloro- 
formed 100th day — increased 270 grams in weight; no lesion of 
tuberculosis. 
Floor main sleeping compartment. 
Sputum exposed and injected into guinea pig. Pig chloro- 
formed 100th day — increased 140 grams in weight; only the spleen 
showed lesions of tuberculosis. 
Control guinea pig died of tuberculosis 60th day. 
+ 
+ 
+ 
% 
