104 
EXPERIMENT NO. 66. 
Autoclave, formalin 2,000 c. c. 
Time required to vaporize formalin, 40 minutes. 
Autoclave changed from one end of car to other every 10 minutes during the process of vaporizing 
the formalin. 
Weather: Clear: very little wind. 
Tempera rare in car at beginning of experiment. 7e.5 : F. 
Relative humidity at beginning of experiment. 64 per cent. 
Time of exposure. 2 hours (11.35 a. m. to 1.35 p. m. from time of beginning to introduce the gas. 
Temperature in car after opening and airing 25 minutes. 83° F. 
Relative humidity after opening and airing 25 minutes. 4} per cent. 
[— means growth: — . no growth]. 
Organism. 
Place of exposure in car. 
Result. 
B. coli communis 
Toilet room 
Smoking room 
— 
Drawing-room 
— 
Floor main sleeping compartment 
— 
Berth, lower No. S 
■ — 
B. diphtherias 
Smoking room 
— 
Dra wine-room 
— 
Floor main sleepine compartment 
— 
Berth, upper No. 9 
4- 
Berth, lower No. 6 
4- 
B. typhosus 
Toilet room 
— 
Smoking room 
— 
Dra wine-room 
— 
Floor main sleeping compartment 
— 
Berth, lower No. 10 
— 
B. subtilis 
Toilet room ' 
— 
Smokine room 
4- 
Floor main sleeping compartment 
4- 
Berth, upper No. 7 . : 
— 
Berth, lower No. 3 
4- 
B. tuberculosus 
Floor main sleeping compartment 
Sputum exposed by Wilson method and injected into guinea 
pig. Pig chloroformed 95th day — increased 410 grams in weight: 
no lesions of tuberculosis. 
Floor main sleeping compartment 
Sputum exposed on carp et and injected into guinea pig. Pig 
died or tuberculosis 59th day. 
Control guinea pig died of tuberculosis 60th day. 
