19 
G. P. No. 58. Six c. c. same serum and injection as g. p. 41. No symptoms. 
[Previous treatment: 21 days prior, inoculated subcutaneously with 0.1 c. c. susoen- 
sion.] 
G. P. No. 44. Six c. c. same serum and injection as g. p. 41. No symptoms. 
[Previous treatment : 2\ days prior, inoculated subcutaneously with 1 gtt. 24-hour 
suspension.] 
G. P. No. 52. Six c. c. same serum and injection as g. p. 41. No symptoms. 
[Previous treatment: 27 days prior, inoculated subcutaneously with 0.1 c. c. 24-hour 
suspension.] 
G. P. No. 50. Six c. c. same serum and injection as g. p. 41. No symptoms. 
[Previous treatment: 32 days prior, inoculated subcutaneous^ with 1 gtt. 24-hour 
suspension.] 
DIPHTHERIA TOXINE CAN NOT RENDER GUINEA PIGS SUSCEPTIBLE. 
For the same reason that guinea pigs were treated with cultures 
of the Bacillus diphtheria we also attempted to render them suscep- 
tible by the injection of small quantities of diphtheria toxine. On 
account of the great importance of this subject a large number of 
guinea pigs were used to demonstrate the role which the diphtheria 
toxine may play in the subsequent action of horse serum. Our 
experiments demonstrate conclusively that diphtheria toxine alone 
plays absolutely no role in rendering guinea pigs susceptible to the 
toxic action of horse serum; for in no instance, as will be seen in the 
following series, does the guinea pig show the slightest effects when 
subsequently injected with large quantities of serum. 
G. P. No. 4584. Six c. c. antitoxic horse serum (Natl. VIII. 17) injected into the peritoneal 
cavity. No symptoms. 
[Previous treatment: 2 days prior, inoculated subcutaneously with 0.001 c. c. toxine 
No. 7.] 
G. P. No. 4593. Six c. c. same serum and injection as g. p. 4584. No symptoms. 
[Previous treatment : 3 days prior, as g. p. 4584.] 
G. P. No. 4534. Six c. c. same serum and injection as g. p. 4584. No symptoms. 
[Previous treatment: 4 days prior, inoculated subcutaneously with 0.002 c. c. toxine 
No. 7.] " 
G. P. No. 4550. Six c. c. 
[Previous treatment: 
G. P. No. 4548. Six c. c. 
[Previous treatment: 
G. P. No. 4585. Six c. c. 
[Previous treatment: 
G. P. No. 4432. Six c. c. 
[Previous treatment: 
G. P. No. 4434. Six c. c. 
[Previous treatment: 
No. 7.] 
same serum and injection as g. p. 4584. No symptoms. 
5 days prior, as g. p. 4534.] 
same serum and injection as g. p. 4584. No symptoms. 
6 days prior , as g. p. 4534.] 
same serum and injection as g. p. 4584. No symptoms. 
6 days prior, as g. p. 4584.] 
same serum and injection as g. p. 4584. No symptoms. 
7 days prior, as g. p. 4534.] 
same serum and injection as g. p. 4584. No symptoms. 
7 days prior, inoculated subcutaneously with 0.004 c. c. toxine 
G. P. No. 4426. Six c. c. same serum and injection as g. p. 4584. No sympto ms . 
[Previous treatment: 8 days prior, as g. p. 4534.] 
G. P. No. 4583. Six c. c. same serum and injection as g. p. 4584. No symptoms. 
[Previous treatment : 9 days prior, as g. p. 4534.] 
