33 
Mairet and Bose a were able to distinguish in blood serum a sub- 
stance capable of producing coagulation and another substance capa- 
ble of producing poisonous symptoms. They believed both of these 
substances to be albumins. They were able to eliminate the coagu- 
lation-producing substance by heating from 52° to 53° C. or by the 
addition of sodium chloride and sodium sulphate. 
Pearce b believes that it appears to be definitely established that 
red blood corpuscle thrombi occur in the experimental lesions caused 
by sera possessing hemagglutinative power. He demonstrated that 
the capillaries of certain areas in the liver are entirely occluded by 
fused red cells, and he also showed the inability of the products of 
hemolysis to produce such action and necrotic areas in the liver. 
L. Camus and E. Gley c first endeavored to separate the hemolytic 
action from the toxic action of blood serum by means of heating. 
They found that heat destroys the hemotytic action; but while it 
diminishes it does not entirely destroy its general toxic action. This 
work was done with eel serum. In their previous work the two 
actions seemed about parallel. For instance, eel serum is very hemo- 
lytic for rabbits and guinea pigs and equally poisonous for these ani- 
mals. On the other hand, the red blood cells of the pigeon and those 
of the porcupine are very resistant to eel serum, and these animals 
also resist doses which are fatal for the rabbit and the guinea pig. 
However, these authors finally found an animal, the marmot, for 
which eel serum had slight hemolytic action but was extremely 
toxic, and they believed that these researches upon the marmot have 
permitted them to separate the toxic action of eel .serum from its 
hemolytic action better than did their previous work by means of 
heating. 
We found horse serum to have practically no hemolytic action 
upon the corpuscles of the guinea pig. This applies with equal force 
to antitoxic horse serum as to normal horse serum, as may be seen 
from the following table: 
Hemolysis. 
G. P. No. 2. Normal pig. Serum obtained by coagulation. 
G. P. No. 1. Normal pig. Copuscles obtained by centrifugation, etc. 
a Mairet and Bose: Recherches sur les causes de la toxieite du serum du sang. Compt. 
rend., t. 119, 4, p. 292. Toxieite du serum du sang de l'homme sain., Compt. rend., 1897. 
Des effects de la chaleur sur la toxieite du serum. Compt. rend. July 7, 1894. 
& Pearce, Richard M. : A further study of the experimental production of liver necrosis 
by the injection of hemagglutinative sera. J. M. Research, v. 14, Apr., 1906, p. 541. 
Pearce, R. M., and Winne, C. K., jr.: Concerning hemagglutins of bacterial origin and their 
relation to hyalin thrombi and liver necroses. Am. J. M. Sci., v. 128, 1904, p. 669. 
c Camus, L., and Giey, E.: Comparison between the hemolytic and the toxic action of 
eel’s serum in the marmot ( Arctomys marmota). Arch, internat. de pharmacodyn. et de 
therapie, tome 15, fasc. 1 and 2, 1905. 
28337— No, 29—06 3 
