40 
oxide (alphozone), butyric acid, hydrogen dioxide, permanganate of 
potash, alcohol, ammonium sulphate, and citrate of soda. 
Butyric acid. 
N 
G. P. No. 4368. Ten c. c. of a Jq butyric acid solution were added to 25 c. c. antitoxic 
horse serum (Natl. IX. 17); the mixture was kept at 15° C. for 40 hours, at the end of 
which time the fine precipitate which developed was filtered off and 3 c. c. injected 
into the peritoneal cavity. Dead in 2 hours 10 minutes. 
[Previous treatment: 30 days prior, inoculated subcutaneously with 0.22 c. c. toxino 
No. 7 + 33-0 c - c - horse serum (Cutter 1461).] 
Hydrogen dioxide. 
G. P. No. 4371. Five c. c. of hydrogen dioxide (Oakland) were added to 25 c. c. antitoxic 
horse serum (Natl. IX. 19); the mixture was kept at 15° C. for 40 hours, at the end of which 
time a very little fine precipitate had formed, but the mixture effervesced in filtering; 
3 c. c. of the filtrate was injected into the peritoneal cavity. Symptoms (?); recovery. 
[Previous treatment: 30 days prior, inoculated subcutaneously with 0.22 c. c. toxine 
No. 7+jyo c. c. horse serum (Cutter 1504).] 
G. P. No. 4917. Five c. c. hydrogen dioxide (McKesson & Robbins) were added to 25 c. c. 
antitoxic horse serum (Natl. IX. 17); the mixture was kept at 15° C. for 40 hours, at the 
end of which time the fine precipitate which developed was filtered off, and 6 c. c. injected 
into the peritoneal cavity. Symptoms; recovered. 
[Previous treatment: 41 days prior, inoculated subcutaneously with 0.22 c. c. toxine 
No. 7+ T +j- c. c. antitoxic horse serum (Stearns 16H).] 
Permanganate of potash. 
G. P. No. 4370. Three c. c. of a 1 per cent solution of permanganate of potash were added to 
25 c. c. antitoxic horse serum (Natl. IX. 19); the mixture was kept at 15° C. for 40 hours, 
and as no precipitate had formed in that time it was not filtered; 3 c. c. of the mixture 
inoculated into the peritoneal cavity. Dead in 45 minutes. 
[Previous treatment: 30 days prior, inoculated subcutaneously with 0.22 c. c. toxine 
No. 7 + 53 - 3 - c - c - horse serum (Cutter 1461).] 
Succinic peroxide acid ( alphozone ). 
G. P. No. 4372. Five c. c. of a 1 per cent solution of alphozone were added to 25 c. c. antitoxic 
horse serum (Natl. IX. 19); the mixture was kept at 15° C. for 40 hours; the precipitate 
which formed was filtered off and 3 c. c. of the filtrate injected into the peritoneal cavity. 
No symptoms . a 
[Previous treatment: 30 days prior, inoculated subcutaneously with 0.22 c. c. toxine 
No. 7+330 c - c - horse serum (Cutter 1504).] 
G. P. No. 5A. Ten c. c, of the same filtrate injected into the peritoneal cavity. Dead in 
35 minutes. 
[Previous treatment: 30 days prior, inoculated with 0.22 c. c. toxine No. 7+1 unit of 
horse serum (Standard B27).] 
As we found that 2 c. c. of a 1 per cent solution of succinic peroxide 
acid (alphozone) injected into the peritoneal cavity of a normal 
guinea pig produced transient irritating effects these experiments 
were repeated, using smaller quantities of the acid, as follows: 
a Explanation of occasional irregularities, p. 63. 
