41 
G. P. No. 4519. One c. c. of a 1 per cent solution of alphozone was added to 25 c. c. antitoxic 
horse serum (Natl. IX. 19); the mixture was kept at 15 C. for 40° hours; the precipitate 
which formed was filtered off and 6 c. c. of the filtrate injected into the peritoneal cavity. 
Dead in 24 minutes. 
[Previous treatment: 28 days prior, inoculated subcutaneously with 0.19 c. c. toxine 
No. 7+1 unit horse serum (Standard B27).] 
G. P. No. 4518. Five c. c. added as above and 6 c. c. of the filtrate injected into the peritoneal 
cavity. Dead in 25 minutes. 
[Previous treatment: 28 days prior, inoculated subcutaneously with 0.19 c. c. toxine 
No. 7+1 unit horse serum (Standard B27).] 
Alcohol. 
G. P. No. 4373. Five c. c. of 95 per cent alcohol were added to 25 c. c. antitoxic horse serum 
(Natl. IX. 19); the mixture was kept at 15° C. for 40 hours; the precipitate which formed 
was filtered off and 3 c. c. of the filtrate injected into the peritoneal cavity. Symptoms; 
recovered. 
[Previous treatment: 30 days prior, inoculated subcutaneously with 0.22 c. c. toxine 
No. 7+++ c. c. horse serum (Cutter 1504).] 
G. P. No. 5B. Seven and a half c. c. added as above and 3 c. c. of the filtrate injected into 
the peritoneal cavity. No symptoms. a 
[Previous treatment: 30 days prior, inoculated subcutaneously with 0.22 c. c. toxine 
No. 7+1 unit horse serum (Standard B27).] 
G. P. No. 156. Seven and a half c. c. added as above and 6 c. c. of the filtrate injected into 
the peritoneal cavity. Dead in 90 minutes. 
[Previous treatment: 60 days prior, inoculated subcutaneously with 0.22 c. c. toxine 
No. 7 + 3 +o c - c - antitoxic horse serum (Stearns 1351).] 
G. P. No. 4363. Ten c. c. added as above and 3 c. c. of the filtrate injected into the perito- 
neal cavity. Symptoms, recovered. 
[Previous treatment: 30 days prior, inoculated subcutaneously with 0.22 c. c. toxine 
No. 7 + 23-0 c - c - horse serum (P D & Co .07173-G).] 
G. P. No. 4C. Twenty-five c. c. added as above. This caused practically solid coagulation 
on the serum. The coagulent was vigorously shaken up with salt solution (0.85) and filtered 
by the aid of a vacuum, and 6 c. c. of the opalescent filtrate injected into the peritoneal 
cavity. Dead in 4 hours 10 minutes. 
[Previous treatment: 30 days prior, inoculated subcutaneously with 0.22 c. c. toxine 
No. 7+1 unit horse serum (Standard B27).] 
Ammonium sulphate. 
We know from the work done with serum precipitated with ammo- 
nium sulphate and subsequently dialysed that the ammonium 
sulphate has no action upon the toxic principle found in horse serum. 
(See guinea pigs Nos. 4104, 4106, and 4513, p. 39.) 
Citrate of soda. 
We know that citrate of soda has no injurious action upon the 
toxic principle found in horse serum because some of the serum we 
used was prepared by adding 1 per cent solution of citrate of soda 
to the whole blood as it was drawn from the horse in order to prevent 
“For explanation of occasional irregularities, see p. 63. 
4 
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