65 
G. P. No. 129. Six c. c. same serum as g. p. 166 mixed with liver of g. p. 109 injected into 
peritoneal cavity. No symptoms. 
[Previous treatment: As g. p. 166.] 
2 hr. 45 min. later. To test susceptibility. 6 c. c. same serum as g. p. 166 injected into 
peritoneal cavity. Dead in 30 minutes. 
G. P. No. 147. Six c. c. same serum as g. p. 166 mixed with brain of g. p. 109 injected into 
peritoneal cavity. No symptoms. 
[Previous treatment: As g. p. 166.] 
1 hr. 20 min. later. To test susceptibility. 6 c. c. same serum as g. p. 166 injected into 
peritoneal cavity. Dead in 35 minutes. 
G. P. No. 139 (control). Six c. c. same serum as g. p. 166 injected alone into peritoneal 
cavity. Dead in 15 minutes. 
[Previous treatment: As g. p. 166.] 
Owing to the fact that guinea pigs 129 and 147 showed no symp- 
toms, the experiment was repeated, using organs from a similarly 
treated pig as follows: 
G. P. No. 108 (immunized). This guinea pig had received 10 subcutaneous inoculations of 
1 c. c. each of normal horse serum (Sam) on 10 successive days from Feb. 8 to 20; 37 days 
later, viz, March 29, it was inoculated with 6 c. c. antitoxic horse serum (Natl. VIII. 18) 
into the peritoneal cavity. Symptoms, recovered. 
Beginning six days later, viz, April 4, and continuing 12 days, it was given a daily sub- 
cutaneous inoculation of 1 c. c. normal horse serum (roan). No symptoms. 
Six days after the last inoculation the pig was given 6 c. c. normal horse serum (roan) 
into the peritoneal cavity. No symptoms. 
This guinea pig, having showed a strong resistance to the toxic 
action of horse serum, was killed with chloroform and the brain, part 
of the spinal cord, and liver were minced and ground separately in a 
mortar with 20 c.. c. antitoxic horse serum (Natl. VIII. 18) . The mix- 
ture was allowed to stand about 3 hours at room temperature and 
strained through cheese cloth and then inoculated. 
G. P. No. 4998. Six c. c. antitoxic horse serum (Natl. VIII. 18) mixed with liver of g. p. 
108 injected into the peritoneal cavity. Dead in 95 minutes. 
[Previous treatment : 39 days prior, inoculated subcutaneously with 0.22 c. c. toxine 
No. 7 + g-jh c. c. antitoxic horse serum (Wellcome 474A).] 
G. P. No. 5044. Six c. c. antitoxic horse serum (Natl. VIII. 18) mixed with brain and 
portions of the spinal cord of g. p. 108 injected into the peritoneal cavity. Dead in 85 
minutes. 
[Previous treatment : 33 days prior, inoculated subcutaneously with 0.139 c. c. toxine 
No. 5 -j- -gTg- c. c. antitoxic horse serum (NYBH 13C).] 
G. P. No. 252. Six c. c. antitoxic horse serum (Natl. VIII. 18) mixed with brain and por- 
tion of spinal cord of g. p. 108 injected into the peritoneal cavity. ■ Symptoms, recovered. 
[Previous treatment: 53 days prior, inoculated subcutaneously with - 2 do c. c. normal 
horse serum (roan) heated to 60° C. for 30 min.] 
G. P. No. 251. Six c. c. antitoxic horse serum (Natl. VIII. 18) mixed with liver of g. p. 108 
injected into the peritoneal cavity. Symptoms; recovered. 
[Previous treatment: 53 days prior, inoculated subcutaneously with 2 x 0 c - c - normal 
horse serum (roan) heated to 60° C. for 30 min.] 
28337— No. 29—06 5 
