81 
Bar and Ren on, ° Picolini and Conti, * * & Mairet and Bosc. c All of these 
authors determined the toxicity of the blood serum in accordance 
with Kummo’s method of intravenous injections into rabbits. 
The poisonous properties of the blood serum from healthy men, 
according to the work of these authors, varies considerably, as may 
be judged from the following summary: 
Rummo found 10 c. c. as the fatal dose for 1 kilogram of rabbit. 
Lugwig and Savor, 8 to 9 c. c. 
Chamb relent and Tarnier, 10 c. c. 
Albu, 9.5 to 11 c. c. 
Mairet and Bose, 12.5 to 18.0 c. c. 
Guinard and Dumarest, 17 c. c. 
Leclainche and Remond, 23 c. c. 
. Charrin, 27 c. c. 
It will therefore be seen that the fatal dose of normal human 
serum, according to these different investigations, varies between 
8 c. c. and 27 c. c. per kilogram of rabbit. 
Uhlenhuth found that the intravenous injection of from 7 to 10 
c. c. of normal blood serum per kilogram of rabbit regularly caused 
death within one-half hour. 
The blood serum of other animals was injected intravenously into 
rabbits and showed considerable differences in their toxicity. The 
quantity necessary to kill in proportion of 1 kilogram of rabbit was: 
c.c. 
Sheep serum 11 
Hog serum . 12 
Cattle serum 6 
Horse serum on the other hand, when injected in such large quan- 
tities as 60 c. c. and more per 1 kilogram of rabbit, not only did not 
kill but caused no reaction at all. 
In 1888 Mosso d discovered that eel serum in doses as small as 
0.02 c. c. per 1 kilogram of rabbit caused death. 
Uhlenhuth, finding intravenous injections into rabbits unsatis- 
factory, used subcutaneous inoculations into guinea pigs. He 
found that the injection of small quantities (0.5 c. c.) of human, 
a Bar and Renon: De la toxicite du sang et de l’urine ehez une femme atheinte de troubles 
gravido-cardiagues. Compt. rend. soc. biol., 1894. 
& Picolini and Conti: Toxicite du serum sanguin et de Purine dans la pneumonie. 
c Mairet and Bose: Recherches sur les causes de la toxicite du serum du sang. Compt. 
rend., v. 119, p. 292. Toxicite du serum du sang de l’liomme sain. Comp, rend., 
1897. *Des effects de la chaleur sur la toxicite du serum. Compt. rend., 1894. 
d Mosso: Die giftige wirkung des serums der muraeniden. Arch. f. exper. pathol. u. 
pharmakol., bd. 25, p. 111. 
28337— No. 29—06 6 
