10 
vehicle by which the suckling organism received its antitoxin and with 
the length of the suckling furnishes it with an increasing immunity. 
The long persistence of the immunity depended upon a transfer of 
antibodies through the milk. By the immunization of a nursing mother 
mouse (after birth of the litter) Ehrlich was able to transmit immunity 
to swine-plague to the nursing young. 
Ehrlich and Hiibener showed that Tizzoni and Centani had erred 
when they appeared to show that in rabbits the progeny of a male 
immune to hydrophobia and a female immune to tetanus was immune 
to hydrophobia. In experi ments with tetanus-immune guinea pigs and 
mice they pbtained results in entire harmony with the previous results 
of Ehrlich; and, opposed to Tizzoni's reports, established that in 
tetanus also no transmission occurs through a male, but that only 
through the mother does transmission of immunity take place which, 
by the end of the second and positively by the end of the third month, 
disappears. 
Xaillard came to the same conclusions from experiments on guinea 
pigs and rabbits. 
The results of Wernicke on diphtheria-immune guinea pigs were 
also the same. ”In diphtheria the immunity is not transmitted by the 
father, but the mother only.” This immunity can not be detected 
in the grandchild, but remains longer in the first generation (the chil- 
dren). for in three months a considerable immunity is still present. 
Transmission of immunity through the milk also occurs in guinea 
pigs. 
Klemperer found in eggs of tetanus-immune hens tetanus antitoxin 
in the yolk, not in the white. 
Kitt injected hens with eggs derived from chicken-cholera-immune 
hens and obtained immunity. 
Sclavo immunized hens against diphtheria by injection of weakened 
cultures, and found that the whites of the eggs protected guinea pigs 
against lethal doses of diphtheria bacilli. 
Tables Nos. 1 and 2 show the results of tests of the young of female 
guinea pigs that had recovered from the effects of an injection of tox- 
ine alone or of the toxine-antitoxin mixture. These animals were put 
aside for breeding after having been under observation 35 da vs after 
injection, which is the time all animals used for antitoxin work in the 
laboratory are kept under observation. 
Table No. 1 shows the results of the tests of the young pigs against 
an L+ dose, and Table No. 2 against an MED. 
