14 
THE EFFECT OF REPEATED INJECTIONS IN THE MOTHER ON HER YOUNG. 
At the suggestion of Doctor Rosenau a number of female guinea 
pigs were given repeated injections either of antitoxin alone or of one 
unit of antitoxin plus slightly more than the L° dose of toxine in order 
to study the effects of repeated injections in the mother on her young. 
An untreated male was placed in the pen with them. The young were 
allowed to remain with their mother until the da}^ before being tested. 
They were tested in most cases against an L+ dose, though in some 
cases a larger dose, either one and a third or one and a fourth times 
the L+ dose, was given. 
Pigs Nos. 4E, 4F, 8G, and 8H received eight and one-third times 
the MLD. 
GUINEA PIG NO. 4. 
May 25, 1905. Given 0.15 c. c. toxin No. 7 (L + .21) -j-1 immunity unit Ehrlich 
15. IV .05. 
May 29. Same treatment. 
June 9. 0.18 c. c. toxine No. 7 + 1 immunity unit Ehrlich 15. IV .05. 
June 16. 0.19 c. c. toxine No. 7+1 immunity unit Ehrlich 15. IV .05. 
June 26. 0.19 c. c. toxine No. 7+1 immunity unit Ehrlich 15. IV .05. 
July 12. 0.19 c. c. toxine No. 7+1 immunity unit Ehrlich 15. IV .05. 
Litter No. 1. 
July 28, 1905. 4 A and 4B born. 
August 25. 4A given 0.22 c. c. toxine No. 7 + 1 immunity unit Ehrlich 15. VIII .05. 
Moderate reaction at site on third day. Recovered. Control died in 3 days 
and 3 hours. 
August 25. 4B given 0.22 c. c. toxine No. 7 + 1 immunity unit Ehrlich 15. VIII .05. 
Died in 6 days and 3 hours. Control died in 3 days and 3 hours. 
Litter No. 2. 
November 7, 1905. 4C and 4D born. 
January 6, 1906. 4C given 0.22 c. c. toxine No. 7+1 immunity unit B27. No reac- 
tion. Control died in 3 days and 7 hours. 
January 24. 4D given 0.293 c. c. toxine No. 7 (=l£ times the L+ dose). Indura- 
tion followed by small ulcer. Recovered. Control died in 1 day and 15 hours. 
Litter No. 3. , 
May 21, 1906. 4E and 4F born. 
June 22. Both given 0.05 c. c. toxine No. 7 (MLD=0.006 c. c. ). Slight induration 
third day. Controls died in 1 day and 4 hours and 22 hours, respectively 
GUINEA PIG NO. 5. 
Same treament as No. 4. 
Litter No. 1. 
July 27, 1905. 5A and 5B born. 
September 9. Both given 0.22 c. c. toxine No. 7 + 1 immunity unit Ehrlich 15. IX .05. 
Slight induration at site in both pigs. Recovered. Controls died in 3 days and 
12 hours. 
