15 
Litter No. 2. 
October 27, 1905. 5C and 5D born. 
January 6, 1906. Both given 0.22 c. c. toxine , No. 7 + 1 immunity unit B27. No 
reaction in either pig. Controls died in 3 days and 7 hours. 
Litter No. 3. 
February 3, 190C. 5E born. 
March 17. Given 0.293 c. c. toxine No. 7 (lj times the L + dose) + 1 immunity unit 
B28. Very slight reaction fourth day. Recovered. Control died in 1 day and 18 
hours. 
Litter No. 4- 
April 12, 1906. 5F and 5G born. 
June 5. Both given 0.275 c. c. toxine No. 7 (1^ times the L + dose) +1 immunity 
unit Ehrlich 15. VI .06. Both showed induration followed by ulcer at site. Con- 
trol died in 1 day and 21 hours. 
GUINEA PIG NO. 6. 
Same treatment as No. 4. 
Litter No. 1. 
September 7, 1905. 6 A' and 6B born. 
October 20. Both given 0.22 c. c. toxine No. 7 + 1 immunity unit B25. No reaction. 
Controls died in 3 days and 2 hours. 
Litter No. 2. 
November 8, 1905. 6C, 6D, 6E, and 6F born; 6C, 6E, and 6F used for other work. 
January 24, 1906. 6D given 0.293 c. c. toxine No. 7 (lj times the L + dose) + 1 
immunity unit B27. Small ulcer. Recovered. Control died in 1 day and 20 
hours. 
Litter No. 3. 
February 17, 1906. 6G born. 
March 17. Given 0.29J c. c. toxine No. 7 (lj times the L + dose). Very slight 
reaction. Control died in 1 day and 18 hours. 
Litter No. 4- 
April 26, 1906. 6H and 61 born. 
June 5. Both given 0.275 c. c. toxine No. 7 (1| times the L + dose). No reaction 
in either pig. Control died in 1 day and 21 hours. 
« 
GUINEA PIG NO. 7. 
May 25, 1905. Given 250 units antitoxin. 
June 9. Given 600 units antitoxin. 
June 16. Given 900 units antitoxin. 
June 26. Given 1,000 units antitoxin. 
July 12. Given 1,500 units antitoxin. 
Litter No. 1. 
July 26, 1905. 7 A born. 
August 16. Given 0.22 c. c. toxine No. 7 + 1 immunity unit Ehrlich 15. VIII .05. 
Small ulcer. Recovered. Control died in 3 days and 7 hours. 
August 24. The mother pig was killed, blood drawn, allowed to clot, and serum 
tested to 12, 25, and 50 units. All the pigs died in about 24 hours, showing that 
her serum contained considerably less than 12 units per cubic centimeter. 
