17 
Sodium chlorid. 
G. P. No. 5341. Six c. c. same serum+0.5 gm. sodium chlorid, subcutaneously. 
Severe symptoms. 
[Previous treatment: 35 days prior, 0.139 c. c. toxine No. 5 + 2 I 0 c. c. antitoxic 
horse serum ( A. A 201 ) . ] 
Magnesium sulphate. 
G. P. No. 5364. Six c. c. same serum+0.2 gm. magnesium sulphate, subcutaneously. 
Dead, 38 minutes. 
[Previous treatment: 35 days prior, 0.139 c. c. toxine No. 5+yoVo c. c. antitoxic 
horse serum (NYBH 306).] 
Ammonium sulphate. 
G. P. No. 5384. Six c. c. same serum -f 0.1 gm. ammonium sulphate, subcutaneously. 
Dead at once. 
[Previous treatment: 28 days prior, 0.139 c. c. toxine No. 5 +Y 2 V 0 c. c. antitoxic 
horse serum (NYBH 305).] 
G. P. No. 7847. Six c. c. normal horse (roan) serum+0.1 gm. ammonium sulphate, 
subcutaneously. Dead, 23 minutes. 
[Previous treatment: 59 days prior, 0.24 c. c. toxine No. 9+g^o c. c. antitoxic 
horse serum (A 192).] 
MISCELLANEOUS SUBSTANCES. 
Ox bile. 
G. P. No. 7616. Six c. c. normal horse (No. 15) serum-fheated ox bile, equal parts, 
intraperitoneally 3 hours after mixing. Dead, 11 minutes. 
[Previous treatment: 45 days prior, 0.142 c. c. toxine No. 5 A 3 I 0 c. c. antitoxic 
horse serum (A. A 248).] 
Animal charcoal. 
G. P. No. 7614. Six c. c. normal horse (No. 15) serum-f animal charcoal; shaken 
up well, filtered, let stand for 3 hours; intraperitoneally. Dead, 20 minutes. 
[Previous treatment: 45 days prior, 0.142 c. c. toxine No. 5+3|^o c. c. antitoxic 
horse serum (A. A 248).] 
Yeast cells. 
G. P. No. 7615. Six c. c. normal horse (No. 15) serum+ground yeast cells; let 
stand 3 hours; intraperitoneally. Dead, 35 minutes. 
[Previous treatment: 45 days prior, 0.142 c. c. toxine No. 5+- c. c. antitoxic 
horse serum (A. A 245).] 
FORMALDEHYD. 
In view of the fact that formaldehyd has a destructive action upon 
such ‘‘haptin” substances as tetanus and diphtheria toxines, and in 
further view of the fact that the sensitizing and toxic principles of 
horse serum seem to belong to the haptin group of substances in the 
sense used by Ehrlich, it became interesting to determine what effect 
formaldehyd would have upon hypersusceptibility produced by horse 
serum. 
Normal serum+S per cent formalin: 
G. P. No. 442. Six c. c. normal horse (roan) serum-|-5 per cent formalin, sub- 
cutaneously. Dead, 12 minutes. 
[Previous treatment: 33 days prior, 0.0006 gm. tetanus toxine A+xtiVti 
toxic horse serum (Hoechst), subcutaneously.] 
3681— No. 36—07 3 
