26 
be interesting to determine whether the synthetic peptids and poly- 
peptids of Fisher sufficiently approach the true proteid molecular 
structure to induce hypersusceptibility in the guinea pig. 
From our work with other proteid substances it was but a logical 
step to the albuminous content of the bacterial cell, which is dealt 
with in another part of this work. 
Hemoglobin versus hemoglobin. 
No. 
G. r. 
First injection. 
Interval. 
Second injection. 
Result. 
411 
0.1 c. c. hemoglobin 
Days. 
22 
6 c. c. hemoglobin, 
Marked symptoms. 
412 
(washed 20 times) 
subcutaneously. 
0.5 c. c. hemoglobin 
22 
intraperitoneally. 
do 
Severe symptoms. 
413 
(washed 20 times) 
subcutaneously. 
1 c. c. hemoglobin 
22 
do 
Dead, 5 minutes. 
414 
(washed 20 times) 
subcutaneously. 
3 c. c. hemoglobin 
22 
do 
Slight symptoms. 
415 
( washed 20 times ) 
subcutaneously. 
5 c. c. hemoglobin 
22 
do 
Very severe symp- 
(washed 20 times) 
subcutaneously. 
toms. 
The hemoglobin was obtained by dissolving the washed red corpuscles 
of a normal horse in distilled water. The red corpuscles for the hemo- 
globin solution used at the first injection, in order to sensitize the guinea 
pigs, was washed and centrifuged 20 times in order to surely wash 
away all traces of serum, the smallest remaining quantities of which 
might have confused the results. The hemoglobin used at the second 
injection was dissolved from red corpuscles washed four times. . 
Egg albumen versus egg albumen. 
No. 
G. P. 
Fir.st injection. 
Interval. 
Second injection. 
Result. 
406 
0.1 c. c. egg albumen, 
Days. 
22 
6 c. c. saturated 
Dead, 30 minutes. 
407 
subcutaneously. 
0.5 c. c. egg albumen, 
22 
solution of egg 
albumen in salt 
solution, intra- 
peritoneally. 
do 
Dead, 18 minutes. 
408 
subcutaneously. 
1 c. c. egg albumen, sub- 
22 
do 
Dead, 25 minutes. 
409 
cutaneously. 
3 c. c. egg albumen, sub- 
22 
do 
Dead, z5 minutes. 
410 
cutaneously. 
5 c. c. egg albumen, sub- 
22 
do 
Dead, 20 minutes. 
732 
cutaneously. 
1 c. c. egg albumen + 
21 
6 c. c. egg albumen 
Severe symptoms. 
salt solution, subcu- 
taneously. 
+ salt solution 
equal quantities, 
intraperitoneally. 
