35 
All the above guinea pigs were subsequently inoculated with a 
virulent culture of anthrax. They all died in a few days with the 
usual lesions. 
A number of guinea pigs were given the extract from anthrax 
bacilli before infection; some were given a single injection, some two 
injections, and others daily injections for twent}" days. Other guinea 
pigs were given the extract used as a vaccine, both in single and 
repeated injections, after being infected with anthrax bacilli. The 
extract did not seem to have an}^ influence on the course of the disease, 
whether given before or after the infection. 
TUBERCULOSIS. 
The indications of hypersusceptibility induced by extract of tuber- 
cle bacilli are restlessness, scratching, irregular respiration, tremor; 
most of the pigs lie down on their sides and look sick. 
No. 
G.P. 
First injection. 
Interval. 
Second injection. 
1 
Result. 
827 
0.1 c. c. extract human 
Days. 
32 
6 c. c. extract hu- ! 
Mild symptoms. 
828 
tubercle bacilli, sub- 
cutaneously. 
1 c. c. extract human 
32 
man tubercle ba- 
cilli, subcutane- , 
ously. 
do 
No symptoms. 
829 
tubercle bacilli, sub- 
cutaneously. ! 
2 c. c. extract human 
32 
do 
Mild symptoms. 
830 
tubercle bacilli, sub- i 
cutaneously. ' 
3 c. c. extract human 
32 
do 
1 
Mild symptoms. 
831 
tubercle bacilli, sub- 
cutaneously. 1 
4 c. c. extract human 
32 
do 
Mild symptoms. 
832 
tubercle bacilli, sub- 
cutaneously. 
5 c. c. extract human 
32 
6 c. c. extract hu- j 
Severe symptoms. 
833 
tubercle bacilli, sub- 
cutaneously. 
16 c. c. extract human 
32 
man tubercle ba- 
cilli, intraperito- 
ueally. 
■ do 
Slight symptoms. 
834 
tubercle bacilli, sub- 
j cutaneously. 
7 c. c. extract human 
32 
10 c. c. extract hu- 
Slight symptoms. 
835 
tubercle bacilli, sub- 
cutaneously. 
1 8 c. c. extract human 
i 32 
man tubercle ba- 
cilli, intraperito- 
neally. 
6 c. c. extract hu- 
INIild symptoms. 
836 
tubercle bacilli, sub- 
cutaneously. 
9 c. c. extract human 
! 
32 
man tubercle ba- i 
cilli, intraperito- 
neally. 
6 c. c. extract hu- 
No symptoms. 
tubercle bacilli, sub- 
cutaneously. 
man tubercle ba- 
cilli, subcutane- 
ously. 
The guinea pigs which have reacted to two injections of proteid 
extract obtained from the tubercle bacillus are now being tested for 
immunity to infection with tubercle cultures. 
