36 
TYPHOID. 
The indications of hj^persusceptibility induced by two injections of 
typhoid extract manifest themselyes by rapid respirations; most of 
the pigs lie down on their sides. The symptoms presented b}^ this 
series of pigs were mild. 
Xo. 
G. P. 
First injection. 
Interval. 
Second injection. 
Kesnlt. 
Days. 
857 : 
10 c. c. typhoid extract, 
subcutaneously. 
84 
10 c. c. typhoid ex- I 
tract, subcutane- } 
ously. ' 
Slight symptoms. 
858 
5 c. c. typhoid extract, 
subcutaneously. 
34 
do 1 
do 1 
Slight symptoms. 
859 
1 c. c. typhoid extract, 
subcutaneously. 
34 
Slight symptoms. 
860 
do 
34 
6 c. c. typhoid ex- 
tract, intraperi- 
toneally. 
Slight symptoms. 
862 
0.5 c. c. typhoid ex- 
tract, subcutane- 
ously. 
34 
10 c. c. typhoid ex- 
tract, subcutane- 
ously. 
Slight symptoms. 
863 
0.1 c. c. typhoid ex- 
tract, subcutane- 
ously. 
34 
do 
Slight symptoms. 
Nine da^^s following the second injection of the extract, tiye pigs of 
the aboye series, which had receiyed 10 c. c. of the typhoid extract at 
the second injection, resisted a large dose of a yirulent typhoid culture. 
Two controls died in eighteen hours. One or two of the pigs which 
had receiyed the extract were slightly sick the following day, but 
the next day had full}' recovered and haye remained so. A definite 
immunit}^ was, therefore, conferred by the two injections of extract 
from the typhoid bacillus. 
TYPHO-LYSIN. 
Along somewhat the same lines etforts to obtain the phenomenon 
of h\^persusceptibility with the dissolyed typhoid bacilli (natural lysis) 
failed. 
G. P. 480W and 550W: 
September 11 to 20, fed dead typhoid culture daily. 
September 26 to 28, fed live typhoid culture daily. 
December 14 to 28, 1 c.c. bouillon culture of typhoid daily, subcutaneously. 
January 15, 10 c.c. heavy old bouillon culture of typhoid, intraperitoneally. 
Killed 1 hour later. Peritoneal contents collected. Then peritoneal cavity 
washed with salt solution. 
G. P. lOOT and 109T: 
September 11 to 20, fed dead typhoid bacilli daily. 
September 28 to November 9, fed live typhoid bacilli daily. 
December 17 to 28, 1 c.c. bouillon culture of typhoid daily, subcutaneously. 
February 19, 6 c.c. heavy old bouillon culture of typhoid, intraperitoneally. 
Killed 1 hour later. Peritoneal contents collected. Then peritoneal cavity 
washed with salt solution. 
