51 
Male (G. P. No. 4530). June 8, 1906. Immune. Placed in cage with above female. 
Previous treatment: 
January 12, 1906. 0.19 c. c. toxine 7 + 1 unit antitoxic horse serum (-B27). 
January 22, 1906. Six c. c. antitoxic horse serum (Natl. VIII, 17) intraperi- 
toneally. Definite symptoms. 
Ditto. Symptoms. 
Ditto. No symptoms. 
Ditto. No symptoms. 
Ditto. No symptoms. 
Ditto (Natl. VIII, 18). No symptoms. 
Ditto. Ditto. No symptoms. 
February 6, 1906. 
February 8, 1906. 
February 14, 1906. 
February 23, 1906. 
March 25, 1906. 
April 18, 1906. 
May .16, 1906. Six c. c. normal horse (roan) serum intraperitoneally. No 
symptoms. 
May 18 to June 27, 1906. One c. c. same serum subcutaneously daily (except 
Sunday), 22 injections. No symptoms. 
January 25, 1907. Six c. c. normal horse serum (horse No. 15). Very slight 
symptoms. 
Offspring-, first litter. One young born December 26, 1906. As soon as born, placed 
to nurse with untreated female (pen 120), whose young were placed to nurse with 
G. P. No. 4426. 
(G. P. No. 4426a.) January 25, 1907. Two c. c. normal horse (No. 15) serum 
intraperitoneally, when 30 days old. No symptoms. 
Young of untreated female P. 120. 
(G. P. No. P. 120a.) January 25, 1907. 35 days old. Two c. c. normal horse 
(No. 15) serum intraperitoneally. No symptoms. 
February 18, 1907. 24 days later. Six c. c. same serum. Very severe symptoms. 
(G. P. No. P. 120b.) January 25, 1907. Same as G. P. No .P. 120a. No. 
symptoms. 
February 18, 1907. Ditto. Dead, 70 minutes. 
Second litter. Three young born March 1, 1907, and not exchanged. 
(G. P. No. 4426b.) March 26, 1907. 25 days old. Two c. c. normal horse 
(roan) serum intraperitoneally. No symptoms. 
(G. P. No. 4426c.) Same treatment. No symptoms. 
(G. P. No. 4426d.) Same treatment. No symptoms. 
These three pigs were again tested April 18 with 4 c. c. of normal 
horse (roan) serum into the peritoneal cavity. All three showed slight 
symptoms, thus proving that immunity, or antianaphylaxis,” was not 
transmitted from the mother. 
FAMILY NO. 10. 
(Immune female; immune male. Exchange.) 
Female (G. P. No. 429). January 25, 1907. Six c. c. normal horse (No. 15) serum 
intraperitoneally. Slight symptoms. 
[Previous treatment: May 18 to June 27, 1906, 1 c. c. normal horse (roan) serum 
daily except Sunday, 22 injections.] 
Male (G. P. No. 4530). June 8, 1906. Placed in cage with female. 
For history of this male, see Family No. 14. 
