56 
death of this guinea pig about 3 c. c. of the peritoneal contents were 
collected and injected into the following sensitized guinea pig: 
Ct. P. xso. 7026. Three c. c. of above fluid into peritoneal cavity. Very severe 
symptoms. 
[Previous treatment: 57 days prior, 0.142 c. c. toxine iS^o. c. c. antitoxic 
horse serum (Ld. 8), subcutaneously.] 
PEKITOXEAL FLUID FROM SENSITIZED GUINEA PIGS INTO NORMAL 
GUINEA PIGS. 
The peritoneal contents were collected immediately after the death 
from 9 sensitized guinea pigs "^hat had received 6 c. c. normal horse 
serum (No. 15) each; 3.5 c. c. of this fluid was injected into the peri- 
toneal cavity of a normal guinea pig, but produced no symptoms. 
After withdrawing the fluid of the peritoneal cavities of the above 
9 guinea pigs the peritoneal cavities were washed with sterile salt 
solution and this fluid injected into the following normal guinea pigs: 
G. P. No. A. — 6 c. c. saline Avashings from aboA'e 9 guinea pigs, intraperitoneally. 
No symptoms. 
B. — 5 c. c. saline Avashings from aboAX 9 guinea pigs, intraperitoneally. 
No symptoms. 
C. — 7.5 c. c. saline Avashings from aboAm 9 guinea pigs, intraperitoneally. 
No symptoms. 
D. — 10 c. c. saline washings from aboA^e 9 guinea pigs, intraperitoneally. 
No symptoms. 
E. — 20 c. c. saline washings from above 9 guinea pigs, intraperitoneally. 
No symptoms. 
F. — 6 c. c. saline Avashings from above 9 guinea pigs, intraperitoneally. 
No symjAtoms. 
G. — 6 c. c. saline AA’ashings from above 9 guinea pigs, intraperitoneally. 
No symptoms. 
RELATION TO METHEMAGLOBIN POISONING. 
The symptoms in the guinea pig somewhat resemble methemaglobin 
poisoning. AVe are indebted to Assistant Surgeon A. i\L Stimson for 
comparative spectroscopic studies of the blood of normal guinea pigs 
and of the blood of susceptible guinea pigs immediateh" after death 
caused by a second injection of horse serum. No methemaglobin was 
found. Onh" the bands corresponding to oxyhemaglobin were seen 
in the blood of the guinea pigs examined immediately after death. 
OXYGEN HAS NO INFLUENCE UPON THE SYMPTOMS. 
A sensitized guinea pig was inoculated with horse serum and at once 
placed in an almost pure atmosphere of oxygen. Another sensitized 
guinea pig (not reinoculated) was placed under the same bell jar as a 
control. The inoculated pig developed s 3 ’mptoms and was dead in 
thirty-five minutes. The control animal showed no unusual manifesta- 
tions after thirty minutes in the atmosphere of oxj^gen. It was then 
given an injection of horse serum and immediately replaced under the 
