58 
THE INFLUENCE OF TETANUS TOXINE UPON HYPERSUSCEPTIBILITY. 
- Besreclka and Steinhart" intimate that guinea pigs sensitized with 
a mixture of tetanus toxine and antitetanic serum are not sensitive to 
subsequent injections of horse serum. These scientists, however, sug- 
gest that their failures on this point may have been due to the small 
amount of horse serum used at the first injection, viz, io¥ov and 
TooVoo C. C. 
We tested some of our used tetanus guinea pigs to determine this 
point and found that tetanus toxine does not apparently influence the 
phenomenon of hypersusceptibility to horse serum. The guinea pigs 
were sensitized with 0.0006 gm. of a dried tetanus toxine, which rep- 
resents 100 minimal lethal doses, plus various amounts of -antitetanic 
serum. All those tested reacted to a second injection of horse serum. 
Tetanus toxine. 
No. 
G. P. 
First injection. 
Interral. 
1 
Second injection. 1 
Result. 
1 
Days. 
438 
0.0006 gm. tetanus tox- 
ine A + c. c. anti- 
toxic horse serum 
(Hoechst) subcuta- 
neously. 
17 
6 c. c. normal horse | 
(roan) serum, in- 
traperitoneally. 
Dead, 30 minutes. 
439 
0.0006 gm. tetanus tox- 
ine A -j- c. c. anti- 
toxic horse serum 
(Hoechst) subcuta- 
neously. 
17 
do 
Dead, 30 minutes. 
444 
0.0006 gm. tetanus tox- 
ine A-hyAo c- c. anti- 
toxic horse serum 
(Hoechst) subcuta- 
neously. 
28 
do 
Very severe symp- 
toms. 
447 
0.0006 gm. tetanus tox- 
ine A (M 2122) 
c. c. antitoxic horse 
serum subcutaneous- 
ly- 
28 
do 
Very severe symp- 
toms. 
451 
0.0006 gm. tetanus tox- 
ine A +Ao(Pk) c. c. 
antitoxic horse serum 
subcutaneously. 
28 
1 
6 c. c. normal horse 
(roan ) serum, in- 
traperitoneally 
(Pasteur Insti- 
tute). 
Dead, 10 minutes. 
1 
440 
0.0006 gm. tetanus tox- 
ine A -|- (Hoechst) 
c. c. antitoxic horse 
serum subcutane- 
ously. 
28 
do 
1 
Dead, 10 minutes. 
THE RELATION OF THE SPLEEN AND THYROID TO HYPERSUSCEPTIBILITY. 
While we believe that this reaction is probabh^ localized in the cen- 
tral nervous S 3 "stem, several experiments were undertaken to deter- 
mine what influence the spleen or the thyroid gland ma}" have upon 
the hypersusceptibilit}" produced by the injection of horse serum. 
«Ann. Pasteur Inst., Vol. 21, No. 2, 1907, pp. 117-127. 
